Analysis Of The Intracellular Proteins From Klebsiella Pneumonia Stressed By Different Heavy Metals

In order to explain the mechanisms of resistance and de-toxic to heavy metal ions in K.pneumoniae, we analyzed the total intercellular proteins from K.pneumoniae using two-dimensional gel electrophoresis after the cells were stressed by different heavy metals (Ag+, Cu2+, Pb2+,Cr3+). The results showed that there were 656 protein spots in the control group, however,458,550,609,558 and 604 spots were observed after stressed by Ag+, Cu2+, Pb2+,Cr3+and Pb2+(Long time), respectively. Of those spots 32,24,17,23 and 37 proteins were up regulated, and 18,8,9,12 and 6 proteins were down regulated when stressed by Ag+, Cu2+, Pb2+,Cr3+and Pb2+(Long time), respectively. Although the most protein expressions were decreased when stressed by different heavy metal ions, there were 31,13,13,16 and 14 of specific expressed proteins appeared after stressed by Ag+, Cu2+, Pb2+,Cr3+and Pb2+(Long time), respectively. These differences indicate the presence of heavy metal ions does affect the intracellular expression of certain genes, and the effect of different heavy metal ions has dissimilar mechanism, so as to further clarify K.pneumoniae on the mechanism of resistance to heavy metal ions has laid a solid foundation of the development of heavy metal ions which has provided a theoretical basis to engineered bacteria with higher tolerance genetically to heavy metal ions.