The Study Of Thiol Compound Respons In Hibiscus Trionum L.on The Heavy Metal Stress

Sulfhydryl compound is an important factor to heavy metal tolerance, accumulation and detoxification in plants,which relate to thiol groups can effectively remove intracellular excessive reactive oxygen species from heavy metal stress metabolic and thiol compounds can generate harmless complexes with heavy metals that;store in particular intracellular organelles about regionalization. However, the coordination she is not very clear at the process of generating low toxicity complexes with the thiol compound and heavy metal ions,because the thiol compound also contains amide group,carboxyl group and other coordinating group. When the plants are subject to different exogenous heavy metal stress, the low molecular weight thiol compounds and protein thiol compounds,response interaction is not clear. So explore the thiol compound of heavy metal stress response mechanism for phytore-mediation of heavy metal pollution and heavy metal detoxification has practical significance.Screen out sulfhydryl-rich Hibiscus trionum L seeds samples with HPLC and the pretreatment method of simultaneous derivatization during cellular wall rupture. The determination results show that, the contents of glutathione(GSH) and total thiols(-SH)in Hibiscus trionum L. seeds are 4.0682 μmol/g and 6.06 μmol/g,RSD are 2.50% and1.17%. Under optimized conditions, recoveries are 100.43% and 101.79%, the detection limits are 0.1593and 0.1854 μmol/g. In this research, thiol-rich Hibiscus trionum L. seeds as biological cell sample,using HPLC determine the variation of glutathione(GSH) and protein thiol(Prot-SH) with soaking Hibiscus trionum L.seeds 2h in different meytal salt solution. Amount of adsorption of heavy metals in Hibiscus trionum L. seed cells that managed by heavy matals is determined by ICP-MC. The results showed that Cu2+ and Hg2+ Stress exausted intracellular GSH and protein thiol as stress response, accompanied by larger intracellular adsorption. The adsorptions are26.9347 μmol/g and 15.8815 μmol/g,RSD are 1.41% and 2.40%。maybe that thoils directly with Cu2+ and Hg2+ action lead to it could not be detected,meanwhile part of the thoil compounds may also be used to remove oxygen radicals through metal ions metabolism in cell. Intracellular Pb2+ adsorption capacUy is 5.5021 μmol/g,RSD is1.49%, and Pb2+ Stress stimulate sample intracellular synthesis of Prot-SH, that increased by 0.8142μmol/g. Maybe Pb2+ combined other coordinating groups of the thoil compounds to reduce the concentration of intracellular Pb2+ at detoxification. AndCd2+ adsorption capacity is very low as 0.0987 μmol/g, while the content of thiol compounds did not cause significant changes. So the response action about to be further explored.This research provides important analytical methods and basic data for further screening biological samplers that are rich in thiol compounds and further exploring thiol compounds response for heavy metal detoxification mechanism.