The Recognition Of Double Deoxyguanosine Triphosphate By DNA Polymerase And Reverse Transcriptase

Virus infecting human body cell tends to replicate rapidly. Virus replication is controlled by its genome. Hence, inhibiting the replication of virus genome is of great importance in virus infection treatment. Nucleic acid inhibits the replication of virus genome, thereby reducing levels of infection, and therefore is widely used for anti-virus treatments. The effect of modified nucleosides on various enzyme needs to be tested and evaluated, which necessitate the establishment of a systematic testing methodology.This paper examines the vitro recognition and incorporation of synthesis double deoxyguanosine acid using Fluorescent Quantitative PCR technology, screen and select lead compounds or nucleic acid that are highly antiviral and establishes a method for testing the incorporation of ribonucleoside acid. Data collected will serve as the basis for future clinical tests.The results show that DNA polymerase recognizes L/D type double deoxyguanosine triphosphate. The replication is inhibited as more double deoxyguanosine triphosphate is incorporated. The type L incorporates ranging from 0.8 mM/L/L ~ 1.6 mM. The replication is further reduced as more Type L is incorporated, and ceases when the incorporation reaches 1.6 mM. The type D incorporates ranging from 1.6 mM/L/L ~ 3.2 mM. The replication is further reduced as more Type D is incorporated, and ceases when the incorporation reaches 3.2 mM. The results indicate that double deoxyguanosine triphosphate can effectively inhibits virus replication. Future research on double deoxyguanosine triphosphate will help explore new anti-HIV and anti-HBV treatments.