Effects Of Lithospermum Erythrorhizon Or Panax Notoginseng Extracts And Gene CG12744 On The Gut Immunity Of Drosophila

Gut immunity is the major immune defense mechanism against foreign pathogens in Drosophila. To observe the effect of the Lithospermum Erythrorhizon and Panax notoginseng extracts in the relief of the Drosophila melanogaster gut injury. Drosophila was treated by sodium dodecyl sulphate (SDS) and Paraquat to observe survival rates. The gut damage model was induced with SDS methods. The number of intestinal stem cells (ISC) and enteroblast (EB) in intestinal epithelium cells were detected by GFP positive cell. Besides, the amount of dividing cells were detected by PhosphoHistone 3 (PH3). We use Dihydroethidium (DHE) and 7-amino-actinomycin D (7-AAD) staining to detected the levels of reactive oxygen species and dead cells. Morphological change of the intestine was imaged by an Axioskop 2 plus microscope. These results show that 10% Lithospermum Erythrorhizon or Panax notoginseng extracts could significantly improve the survival rate of SDS and Paraquat caused intestine damage of D. melanogaster. These results suggest that 10% Lithospermum Erythrorhizon or Panax notoginseng extracts could significantly inhibit the overproliferation of progenitor cells, reduce the death number of intestinal epithelial cell, with statistical difference; lower the levels of reactive oxygen species; protect and maintain the intestinal morphology. These results demonstrate that the extracts from Lithospermum Erythrorhizon or Panax notoginseng could significantly enhance the intestinal immune function of D. melanogaster as well as ameliorate and protect intestinal immunologic function injury induced by SDS and Paraquat.The decline in stem cell function caused by aging could lead to many diseases. To study the function and mechanism of CGI2744 in the gut immune system of D. melanogaster, the expression of CG1744 in different age were detected by semi-quantitative PCR. Drosophila was treated by Paraquat, SDS and NaCl to observe survival rates. The amount of dividing cells were detected by PH3. Besides, The number of progenitor cells in intestinal epithelium cells were detected by GFP positive cell. These results suggest that the expression of CG12744 increased in aging processes. The survival of CG12744CE13306 and CG12744RNAi flies is significantly reduced compared with controls following ingestion of Paraquat. CG12744 low expression mutant could significantly cause the overproliferation of ISCs with progenitor cells. The discovers were provided important theoretical basis for the study on the funtion of CG12744 in gut immune.