| Microbial electrolysis cell(microbial electrolysis cell MEC) is a new type of electrochemical biological technology in which less energy can be input, but generates and recycles hydrogen or Methane from the internal reactors by using specific microorganism in the substrate of macro-molecule argon matter decomposition.Although microbial electrolysis cell has many application prospects, there are still some problems which are the amplification of reactors, con-figurative optimization of reactors, condition optimization of reaction and how to filter the dominant strains of producing electricity and gas production and whether the effect is good or not. By doing some research work and literature, it is found that using different gases can affect the results of Hydrogen production of reactors in the Oxygen aeration process of microbial electrolysis cell. Photo fermentation bacteria can also generate Hydrogen under anaerobic conditions using small molecule organic matter. Therefore the purpose of this experiment is to explore the replacement of different aeration gas and observe the changes of the properties of Hydrogen production reactor and the effect of micro electrolysis by adding Photo fermentation bacteria.This study is to use single chamber microbial electrolysis cell(MEC) reactor,respectively studying how head space gas affects the properties of Hydrogen production through the environment for non photosynthetic MEC and join the Photo fermentation bacteria A7(Rhodopseudomonas sp. nov. strain A7,GenBank No. KJ699180) after photosynthesize cultured MEC. Head space gas here, refers to the reactor using different gas in Oxygen aeration process and the remaining gas after ending of the aeration above the solution inside the reactor,the remaining gas called for the head space gas inside the reactor.It is found that the MEC reactor has yielded more Hydrogen in the presence of argon by the experiments and the yield of hydrogen is up to 2.24 mol-H2/mol-acetate.The yield of Hydrogen production in the Argon of non-photosynthetic MEC is the highest but the yield of mixture of Carbon dioxide and Argon of non-photosynthetic MEC is the lowest, which is down to 1.49 mol-H2/mol-acetate.This shows that different head space gas does have an effect on the Hydrogen production of the reactor.By high-throughput sequencing, the result shows that the performance of Hydrogen production is affected by the concentration of Geobacter which is located on the anode carbon brush. The concentration of Geobacter is higher in Argon of non-photosynthetic MEC than that of Nitrogen of non-photosynthetic MEC. Under the same condition of photosynthetic and non-photosynthetic MEC, it is found that the concentration of Photo fermentation bacteria is higher than that of Geobacter.It is found that the MEC reactor has yielded more Methane in the presence of the mixture of Carbon dioxide and Argon by the experiments and the yield of Methane is up to 0.394mol-CH4/mol-acetate. The yield of Methane production in the mixture of Carbon dioxide and Argon of non-photosynthetic MEC is the highest but the yield of of Nitrogen photosynthetic MEC is the lowest, which is down to0.183mol-CH4/mol-acetate.This shows that different head space gas does have an effect on the Methane production of the reactor.By high throughput sequencing, it is concluded that the performance of the methane producing is affected by the concentration of Methanobrevibacter. Part of the Carbon dioxide can provide a good environment for the growth of methane producing bacteria,so the photosynthetic and non photosynthetic MEC containing Carbon dioxide has the best methane production performance. |
