Synthesis, Characterization And Application Of Molecularly Imprinted Mesoporous Silica And Restricted Access Materials

Molecularly imprinted mesoporous silica （MIP-MS）, restricted access media-molecularly imprinted polymer （RAM-MIP） were prepared based on self-assembly techniqueand sol-gel process. Their characterization and application had been investigated. This thesisconsists of four chapters and as follows:Chapter one: Research developments for the synthesis methods and the applications ofMIP-MS and RAM-MIP in separation science were reviewed in the paper.Chapter two: Based on double-imprinting concept and self-assembly technique, a newmolecularly imprinted mesoporous silica （MIP-MS） was prepared, this material was preparedby using enrofloxacin and surfactant micelles as the templates formed through self-hydrolyzed,self-condensed, and co-condensation of tetraethoxysilane （TEOS） and methyltriethoxysilanein alkaline media, and with the covalently anchored organic groups in the mesoporous silicamatrix. The adsorption mechanism was characterized by equilibrium study, kinetics studiesand the competitive adsorption. The kinetics studies showed that the pseudo-second-ordermodel was consistent with the kinetic data of the MIP-MS with the fast binding templates.The equilibrium data showed that the Fundlich model was found to fit the experiment datawell. The thermodynamics parameters indicated that the adsorption process wereentropy-gained, exothermic, and the spontaneous, the competitive adsorption shown thatMIP-MS have high selectivity for the template of enrofloxacin.Chapter three: A new synthesis method of cefotaxime sodium-imprinted mesoporoussilica （MIP-MS） was developed for selective solid-phase extraction （SPE） of cephalosporinsantibiotics residues in milk samples. Kinetic studies show MIP-MS has a goodadsorption-desorption performance. Under the optimal SPE condition, the recoveries ofcefpirome sulfate, cefotaxime sodium, and cefuroxime sodium were reached83.83.-92.60%,85.57-95.18%and85.23-90.89%, respectively, with relative standard deviations lower than7.10%. The limits of detection （LOD） of the proposed method （S/N=3） were in a range of3.1-7.10g kg^-1, and the limits of quantitation （LOQ, S/N=10） were in a range of10.3-23.7g kg^-1.Chapter four: Anew restricted access media-molecularly imprinted polymer （RAM-MIP）was developed based on self-assembly and sol-gel technique. RAM-MIP was prepared byself-hydrolyzed, self-condensed, and co-condensation of TEOS and （Octyl）-trimethoxysilane in the presence of lomefloxacin and surfactant micelles. The γ-Glycidoxypropyltrimethoxysilane （KH560） was used for hydrophilic surface modification. Analysis of FT-IRspectra and TG Curves showed that organic groups were bound in the inner and outside of theRAM-MIP; the recognition properties and hydrophilic properties of RAM-MIP wereevaluated by state adsorption and swelling study, the recovery of BSA by RAM-MIP washigher than99%, this supply that RAM-MIP has good exclusion properties to macromolecule.