| High performance liquid chromatography (HPLC) has been an important analysismethod, and column is the core of the HPLC. The monolith column is a new kind of column,which has the characteristic of high performance and low pressure as well as permeability,moreover, can be modificaed to different function column. So monolith column has been theemphasis and hotspot in the field of the HPLC.First, this paper reviewed the research progress of the monolith column.Second, a new monolithic column was prepared with N-isopropylacrylamide (N-IPAAM)as the monomer and ethyleneglycoldimethacrylate (EDMA) as the cross-linker. Then thestructure of monolith column has been charactered. Then the monolith column was used toenrich hydrocortisone in urine sample, and then linked the C18column for analysis. Theresults showed that this kind of monolith column could be used to extract the hormone drugand remove the biomacromoleculer in urine samples. In all, it is a fast, simple, effectivemethod for assaying and analysis the hormone drugs in urine samples.At last, γ-aminopropyltriethoxysilane (HG-550) was chosen as the chain extenders toopen the epoxy groups of the epoxy resin. Then, a new biphenyl saline with long organicchain was successfully acquired, furthermore, tetraethoxysilicone (TEOS) was added and thereaction of dehydration condensation was finished. The result was a biphenyl salinemonolithic column obtained, which has good pore structure and mechanical strength.Importantly, the new monolithic column jointed with C18column was used to enrich andanalyze the betamethasone in urine of human. |
PDF Full Text Request