Transcriptome Comparison Of Four S.erythraea Strains And Nitrogen Metabolism Analysis

Genetic engineering of Saccharopolyspora erythraea and the molecular mechanism of erythromycin biosynthesis are essential to improve the erythromycin production. On the other hand, both of them can improve the new derivatives, which also can explore the stain’s others physiology characteristics.In this study, one wide-type strain NRRL23338and three kinds of industrial strains which product erythromycin dramatically were compared on the transcriptome level by gene chip. The results showed that there are212and745significantly changed expression genes in both three industrial strains at two time points respectively(30h and40h). Among those genes, many of them belonged to the nitrogen metabolism such as glnR and glnB. However, some other genes were related to the transport proteins of ammonium salt and oligopeptides, which were significantly depressed in the three industrial strains.Based on the results of this study and some other research reported, the nitrogen metabolism has close connection with the erythromycin biosynthesis in Saccharopolyspora erythraea. What’s more, different nitrogen resource has different metabolism pathway in the wild and industrial strains, and influences on the antibiotics production. The metabolism products (organic acid and amino acid) levels of two kind strains were also investigated in this work. With the finding that global nitrogen regulation factor glnR is dramatically repressed in industrial stains, we make efforts to knock it out to evaluate its regulation rules and impact on erythromycin biosynthesis.