| Gold nanoclusters（AuNCs）, as a new t ype of na nomate ria ls wit h uniqueoptica l pr operti es, have attra cted a gre at deal of attenti on fr om researcher srecent years. Gold na nocl us ters have grea t a pplica ble prospe ct in t hebiologica l i magi ng and optical sensing owing to t heir l o w t oxicit y, e xcel l e ntbiocompatibility, stability, so lubility and fluorescene. In t he present work,water-sol uble AuNCs a nd Au/AgNCs wer e synt hesi zed a nd used asfl uore sce nce pr obl es t o de te r mi ne copper（Ⅱ）, l ead（Ⅱ） and ni ckel（Ⅱ） i o nsin a que ous s oluti on. The mai n re searc h content s are summarized as follows:（1） The water-sol uble gol d na nocl uster s that capped with bovine serumalbumi n（BSA） a n d c hicke n e gg al bu min （CEA） were synthesi zed andcharacteri zed by ul traviol et/visibl e spectrum, fluorescence spectr u m andFT–I R spectrum, r espectivel y. The y both e mi tt ed int ens e red fluoresce nceunder UV light（365nm）. The influe nce of rea cti on ti me, t he c once n trationof albumi n and t he pH of t he reaction soluti on on the fluorscence i n tensit yof t he nanoclusters were inve stigate d.（2） A fast and si mple met hod for the detection of copper（Ⅱ） i o ns wasdeveloped based on the fluorescence quenching of gol d nanocl ust e rs whichwere synt hesiz ed by bovi ne serum al bumin （BSA-AuNCs）. The linear rangeof5.0×10-51.0×10-3mol/L and1.0×10-101.0×10-7mol/L was obtainedwith a detection limit of0.05nmol/L.（3） The facts of infl uence on the fl uorscence intensity of interactionbet ween l ead（Ⅱ） io ns an d BSA-Au NCs were i nvestigated, and the optimu mconditions were deter mi ne d. Under opti mum condit ions, t he relati vefl uorescence i ntensity of BSA-AuNCs was linearly proportional to theconc entra tion of l e ad（Ⅱ） i o ns i n t he range fr om0.5t o10μmo l/L, wit h adetecti on li mit as l ow as0.1μmol/L. The me cha nis m of t h e pr opose d a ssa yfor t he detection of lead（Ⅱ） ions ha s a lso bee n discus sed i n detail.（4） CEA was utilized as the reduction agent and compound capped in thesynt hesi s of wat er-soluble gold/si ver alloy nanoclusters（CEA-Au/Ag NCs）.The y e mit ted inte ns e or ange fl uore sce nce under UV light and the qua ntumyield was as hi gh as9.2%. We have fo und that nickel（Ⅱ） ions coul d enha nce t he upconversi on fl uor escence of t he CEA-Au/AgNCs. A novelfl uorescence sensor for nickel（Ⅱ） i ons det ec tion bas e d on upc onvertionfl uore sce nce of CEA-Au/AgNCs ha ve been develope d for t he fi rst ti me.Usi ng t his probe, aqueous nic kel（Ⅱ） i o ns c oul d be dete cted at0.1nM base dfl uore sce nce enhancing in a fa cile wa y, wi th hi gh sele ct ivity against othermet al i on s. The r esearch provided a new idea for t he analytical detectionapplic ations of gol d na nocl ust ers. |
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