Study On Cyclotella Asterocostata-microcystis Aeruginosa Vernalization Mechanism

2011,2013, experiments have confirmed the Cyclotella asterocostataandMicrocystis aeruginosavernalization,itsvernalization control mechanism proposedstudy aims to analyze vernalization control model at the genetic level.By analyzingmacromolecules biological characteristics of microalgae, affected by temperature ofmacromolecules, the effect of temperature on the enzyme, plasminogen activationprocess, the principle of single-celled organisms such as genetic manipulation, geneticcontrol mechanism of microalgae vernalization, and come to the following severalconclusions.1）The fuction ofregulation of protein kinase: activation of plasminogen on theprinciple, the regulatory protein itself does not have an active role in fibrinogen, it needsto regulate the activation of protein kinase, the protein having the activity of aregulatory action, and regulatory proteins then combined with the manipulation of genes,open the whole operon.2）Effect of regulating protein kinase function by temperature: Regulation ofprotein kinase itself is a temperature sensitive protein, the effects of differenttemperatures, will affect the function expression. Temperature sensitive regulation ofprotein kinase in the growth temperature, dormancy under high temperaturedenaturation, enzymatic protein denaturation and renaturation by low temperatureeffect.3）The location of activation gene: Activation of gene and control gene linkage isactivated genes in the DNA position location, activating genes located in themanipulation of genes to a while ago, and manipulation of gene structure, gene operonchain together, play a major role in the manipulation of genes is the geneticmanipulation system control.4) The control of manipulation gene and activation gene: Regulatory proteins oftenform and combination of genetic manipulation is saturated with the gene structure, theworking efficiency is maximum; activate gene transcription regulation of open compilerprotein kinase; normal regulatory protein and chain combination of geneticmanipulation is not combined with active gene, does not affect the normal transcriptionand translation of gene activation therefore, the normal generation will not affect theregulation of protein kinase 5) The working principle of activating gene and control gene chain operatingmodel: spring, regulating protein gene to produce original regulated protein kinaseactivation of regulatory proteins, and the combination of genetic manipulation, startingstructure synthesis of DNA synthetase gene transcription, DNA replication is complete,algae fission (reproductive) population growth. Summer, sensitive regulatory proteinactivating enzyme denaturation in high temperature, adjust fibrinogen activation ofallosteric regulatory protein, and manipulation of genes and activate gene combination,activate gene cannot produce regulating protein kinase activation of fibrinogen toproduce activated protein, then enter the growth of Microcystis aeruginosawas inhibited,Cyclotella asterocostata into high temperature dormancy. Enters the autumn, because notemperature before the thermal denaturation of activating enzyme complex, activationof the normal regulatory proteins bind to the operator, starting structure gene replicationrequired DNA enzymes, although autumn temperature light suitable, Cyclotellaasterocostata won’t grow, Microcystis aeruginosagrowth but the growth rate lower thanthe growth rate the summer at the end of spring; into the winter, when the lowtemperature heat denatured activated enzyme renaturation, wait for next year, activationof fibrinogen, the launch of a new year’s growth.According to the theory of zymogen activation principle, microalgae breeding,gene control model, the temperature influence on biological macromolecules, andcombined with experiment data, finally come to activate gene and control gene linkagecontrol model. On this basis, the microalgae vernalization mechanism was explained, itexpounds two kinds of algae outbreaks in late spring and early summer.