Study On Isolation And Degrading Characteristies Of Triadimenol-degrading Bacteria

Bioremediation mainly by microbial biodegradation of organic pollutants fromthe environment into non-toxic harmless or less toxic substances, with higheradvantages that physical and chemical remediation can not be compared, is one of thebest methods so far to deal with the polluted water and soil effects.Triadimefon,triadimenol and other triazole fungicides are widely used on fruits,cereals and othercrops.The main metabolite of triadimefon is triadimenol. Triadimenol is very difficulthydrolysis at pH4-pH7and occurs mainly biodegradation in soils,degradation half-lifeis110-375days in sandy loam and240-270days in loam soil, are more difficultydegradation pesticides.Research of triadimenol degradation in soil have been a fewreports, triadimenol degrading bacteria screening in this paper and preliminary studyof triadimenol enantiomers elective behavior in soils were first reported and haveimportant significance.This article through to enrichment culture soil sample from a Auto Parts City inHangzhou and a pesticide plant in Jiangsu province, screening degrading bacteria formany times, bacteria no.10,12,43,and49have high efficient degradation,thedegradation rate of triadimenol were29.80%,43.23%,22.89%and29.80%respectively at5d in ordinary medium.Biochemical incubator for10days,degradation of triadimenol of bacteria10and12in the red soil is very obvious, therate of degradation is80.64%and92.11%when triadimenol concentration is5mg/kgin the soil;bacteria43and49obvious effect on the degradation of triadimenol influvo-aquic soil, the degradation rates are66.71%and63.67%. No.10,12,43,49wereidentified were Bacillus fusiformis,Bacillus subtills,Lysinibacillus xylanilyticus andLysinibacillus sphaericus.This paper had three kinds of soil, fluvo-aquic soil, paddy soil and red soilfor the study of triadimenol degradation situation, at the same time set on thesterilization. Column with high performance liquid chromatography (HPLC) as thefoundation to detecte the residues. In order to determine the degradation effect ofbacteria in the soil,adding bacteria in three kinds of soil for triadimenol degradationexperiments, the results show that when the triadimenol concentration was25mg/kgin soil samples,Bacillus fusiformis and Bacillus subtills could shorten the degradation half-life of triadimenol about100d and96d in red soil. The degradation half-life oftriadimenol could shorten about89d and102d in red soil after addingLysinibacillus xylanilyticus and Lysinibacillus sphaericus, and about43d and44d inpaddy soil.Its known from the analysis of degradation data,Lysinibacillus xylanilyticus has overall effect for triadimenol degradation in paddysoil, Lysinibacillus sphaericus has overall effect in the red soil and can significantlyshorten the degradation half-life of several enantiomers of triadimenol.