Study On High-through Detection Of Foodborne Virus

As one of the basic indispensable elements to the survival of mankind, food isclosely related to human life. To enhance its quality and safety management and toprevent food contamination is to avoid unpredictable human health hazards. The studyfound that the food-borne virus can cause diseases on gastrointestinal tract, liver andnervous system, and these diseases are prevalent and infectious. Once break out, itwill lead to serious consequences. The outbreak of food borne viral diseases not onlyposts a threat to a country’s peace and prosperity, and also hinders the country’seconomic prosperity and development in particular food-related industries. Therefore,the research of food-borne virus detection promotes the further development of foodquality and safety systems, and even has deeply impact the prevention and control ofviral food borne illness far-reaching. The main traditional detection methods offood-borne virus are observations directly through microscope, cell culture, andimmunological detection. These methods are experimental procedures that arecumbersome, time-consuming, laborious and easily causing errors. Therefore, as moreand more people lucubrate on the study of modern molecular biology techniques, itgives birth to a new type of fluorescent multiplex real-time RT-PCR technology whichis the improvement of traditional RT-PCR technique. The new born technique bringshope for the realization of fast and accurate virus detection of food.Because there are a large number of pathogens and complex detections, thisstudy combine lock nucleic acid technology come up with a method can detect norvirus, Sapp virus, rotavirus, atrocious, adenovirus and enter virus include poliovirus,coxsackievirus, echovirus in total six species and twenty-one serotypessimultaneously. The method possesses a lot of advantages including excellentsensitivity, specificity, reproductivity. On the one hand it can monitor foodborne viralepidemiology, on the other hand screen various foodborne viruses at the same time.All of these provide powerful science guarantee and essential theoretics foundation tosupervise foodborne pathogens, moreover provide necessary theoretical guarantee andrelevant technical supports to passenger liner economy.