Study On The Pretreatments Of Microemulsion Combining Ionic Liquid On The Enzymatic Hydrolysis Saccharification Efficiency Of Woody Biomass

Biomass resource is the most abundant renewable resources on the earch, its reserves isjust less than coal, petroleum and natural gas. With the fossil fuel becoming exhausted, theresearch and application of biomass fuel and platform chemicals have got highly attentionfrom domestic and overseas experts. In order to avoid causing any food scare from theoveruse of core as bioenergy raw material, people realize the lignocellulose is a more suitablechoice than food crops, the effective improvement of its compact internal structure bypretreatment can lead to the high efficiency of enzymolysis process. Due to theshortbacks(high energy consumption, high pollution, not environmental friendly) oftraditional pretreatment method in the preparation process of bioenergy, a new kind of way isneed to be explied to solve this bottleneck problem.New microemulsion systems including SILMS and DILMS was prepared by replacingthe oil and water phases with [BMIM]Cl and [BMIM]PF6in in the laboratory. ComprehensiveCharacterizations were conducted and analysed effectively, which included pseudo-ternaryphase diagram, grain size, conduction, surface tension and so on. The imprenation mechanismof SILME and DILMS for lignocellulose was studied deeply by the comparison amongSILMS, DILMS, conventional solvents(water, n-butyl alcohol) and traditionalmicroemulsion(O/W and W/O). Pretreatment reaction was conducted with the method ofquadrol impregnation+DILMS treatment, the physical properties of powder like functionalgroup, crystalline texture, thermostability and microstructure were detected and analysed byFT-IR, XRD, TG-DTG and SEM detection means. Eucalyptus powder was treated with themethod of enzymolysis (acidolysis), the effect of treatment method, proportion of components,temperature and mesh number on the efficiency of pretreatment were reflected by thecomparison of glucose yields before and after pretreatment, and the yields were calculated byion chromatography means.The experimental results showed that the new ionic liquid microemulsion systems wereprepared successfully in all detection means of physical properties, including degree ofdispersion, range of grain size, thermostability, conductition and so on. To be different fromconventional solvent, the impregnation regular of SILMS and DILMS for wood was enduring and uniform, the final saturated impregnation quality of SILMS and DILMS were muchhigher than others’. Especially under the condion of80℃,400min, the saturated impregnationquality of DILMS for eucalyptus can reach to132%. Some properties of powder gotimproved after composited pretreatment, hemicellulose were partly removed, the net structureof hydrogen bond in cellulose was destroyed effectively, Specific surface area and quantity ofhole and wrinkle on powder got increased, compactness of structure and thermostability gotdecreased, all these improvements can do contribution to the increase of enzymolysisefficiency. Under the condition of solid-to-liquid ratio1:30, mesh number40, quadrolimpregnation(70℃，3h) and DILMS treatment(100℃，8h), the Enzymatic hydrolysis yield ofglucose after pretreatment was2.21times than blank experiment’s, which proved that theenhancement effect of pretreatment process was really obvious.