A Study Of Building Biomembrane On The Self-assembled Film Patterned By Plasma

With the invention and improvement of microscope, the cell was found. For any life, a cell is its basic structure and functional unit, and all the cell membrane commonly known as a biofilm. Biomembranes are the essential structures for the life activities, which divide a cell into compartments to make the genetic material and other related biological macromolecular in a relatively stable environment. It arouses great interests to separate and study the structure and function of membrane proteins in a lipid bilayer environment. Recently, artificial lipid membranes are used to study the structure, property and functions of biomembranes. Generally the distance between the substrate and the phospholipid membranes is about1~2nm, which causes a difficulty to reconstitute transmembrane proteins and the decrease of membrane fluidity. Hence, it is necessary to build lipid bilayers far away from the substrate, so that it is suitable for reconstitution of transmembrane proteins. This new type of model membrane is also more similar to real biomembranes due to the minor influence from the substrate.In this thesis, we formed the biomimetic membrane arrays standing far away from substrates through two different methods based on the patterned ODS (Octadecyltrimethoxysilane) and APTES (3-ammonia propyl triethoxy silane) self-assembly membranes using plasma oxidation. The polyelectrolytes were layer-by-layer assembled and the gold/silver nanoparticles were assembled on the ODS and APTES membranes, respectively. As a result, the groove structure of micron arrays, which depth can be tuned between5nm~150nm, was built. In the end, the giant phospholipid vesicles (10~100m in diameter) prepared by electric forming method ruptured and spread on the microwell arrays to form lipid bilayer arrays far away from the substrate. Details are as follows:1. After the slides were washed by deionized water and ethyl alcohol, its surface was cleaned and hydroxylated by plasma, and the ODS and APTES assembly monolayer was formed on the surface. The PDMS stamp with groove structure was printed on the surface of ODS and APTES accompanied with plasma oxidation for1min to get the patterned surface.2. The groove structure was constructed by the alternate deposition of polyelectrolyte PDDA and PSS on the patterned ODS self-assembled membrane. The nanoparticles was absorbed onto the patterned APTES surface.3. Giant phospholipid vesicles (10~100m in diameter) were prepared by electric forming method. Then the base with groove structure was immersed into the solution of phospholipid vesicles and the vesicles would rupture and spread on the microcell arrays to form lipid bilayer arrays far away from the substrate.