Low Energy Emulsification Nano-emulsions And As Astaxanthin Carried System

Nanoemulsion has droplet with mean radius between10nm to100nm.Nanoemulsions can increase the bioavailability of insoluble substances and theirresistance to environment stress. The formation of nanoemulsion is not spontaneous,the system need input energy. They are usually prepared by high-energy methods, thatis, applying a mechanical energy input. Nevertheless, emulsification can also beachieved by low-energy methods, which take advantage of the physicochemicalproperties of the system.To screen the best formulation of nanoemulsion, this study choose LabrafacLipophile WL1349, Olive Oil, Labrafac CC, Labrafil M1944CS, Lauroglycol-90asoil phase,and Cremphor ELP, Labrasol, Tween20as surfactants. The results showthat the best formulation is Labrafil M1944CS/Cremphor ELP/water. Thephysicochemical properties of the nanoemulsion is investigated by dynamic lightscattering and transmission electron microscopy. The results show that droplets ofblank nanoemulsion investigated by Mastersizer were depend on the ratio of oil andsurfactant，finally, milky white oil in water (O/W) nanoemulsions have been formedin the system Labrafil M1944CS/Cremphor ELP/water (6:4:90) by using lowenergy emulsification routes, with droplet is64nm, based on the skin penetration andthe practical appliance. Blank nanoemulsion had good stability in a week, and isspherical structure in the field of transmission electron microscopy.The astaxanthin oil (containing5%astaxanthin) and mixed with acid esters ofpolyethylene glycol, Labrafil M1944CS, prepare nanoemulsion loaded astaxanthin.Investigate the stability of astaxanthin in different temperatures, the results showedthat the higher the temperature increased the instability of astaxanthin, at4Centigrade,astaxanthin retained87%, at25Centigrade, astaxanthin content remained77%, at37Centigrade, astaxanthin content remained68Centigrade after a week placed inincubator. Nasopharyngeal carcinoma cell assay employing astaxanthinnanoemulsion，the results show that when the concentration of astaxanthin reach30μg/ml, can significantl inhibit the proliferation of cancer cells. Wrap macromolecules on asta anthin nanoemulsion to stud the effects of macromolecules’biological function on astaxanthin stability, the results show that under55Centigrade,after a week place in incubator, the content of astaxanthin in hyaluronic acid wrappedastaxanthin nanoemulsion also retained68%the content of astaxanthin in caseinwrapped astaxanthin nanoemulsion retained58%, the content of astaxanthin inchitosan wrapped astaxanthin nanoemulsion retained34%, indicating that thehyaluronic acid astaxanthin nanoemulsion improves the heat resistance of theastaxanthin. Use the abdominal skin of mice to measure astaxanthin nanoemulsionskin permeability, and to determine the effect of biological macromoleculesastaxanthin nanoemulsion transdermal effects, the results suggest that as a controlnanoemulsion, casein nanoemulsion astaxanthin, chitosan astaxanthin nanoemulsion,hyaluronic acid nanoemulsion astaxanthin transdermal rates ere0.378μg/cm2.h,0.383μg/cm2.h,0.766μg/cm2.h,0.874μg/cm2.h. This result shows the effect ofhyaluronic acid can enhance penetration speed of astaxanthin nanoemulsion.