Synthesis Of Nanoscale Coordination Polymers Using1,1’-dicarboxyl Ferrocene As Building Blocks And Research On Their Applications In Multifunctional Magnetic Resonance Imaging

Coordination polymers，a class of hybrid materials formed by the self-assembly ofpolydentate bridging ligands and metal-connecting points, have been widely appliedin chemistry, physics and related interdisciplinary ifelds. Recently, nanoscalecoordination polymers (NCPs) have opened up a new avenue ofr biomedicalapplications due to their structural and chemical diversity, high loading capacity, andtheir intrinsic biodegradability. In this thesis, two types of NCPs were synthesized andapplied in multifunctional magnetic resonance imaging (MRI). The work includingtwo parts as following:1. Synthesis of Fc-Gd@SiOi(RBITC)-RGD NCPs for multifunctional magneticresonance imagingIn the present study, we develop Gd-based nanoscale coordination polymers(NCPs) for targeted dual-mode T\-and^-weighted MR imaging on a singleinstrument in vivo. Firstly, stable Fc-Gd NCPs were synthesized by l，l’-dicarboxylferrocene as building blocks and Gd(III) ions with highly paramagnetic properties asmetallic nodes using a nanoprecipitation method. The silica-coated NCPs wereaminated through silanization, which enabled futrher covalent conjugation of afluorescent rhodamine dye (RBITC) and RGD peptide onto their surface for targetedimaging in vitro and in vivo. The formed Fc-Gd@Si02(RBITC)~RGD NCPs werecharacteirzed by transmission electron microscopy (TEM), scanning electronmicroscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and relaxivitymeasurements. The targeting speciifcity of Fc-Gd@SiC>2(RBITC)—RGD NCPs wereevaluated by T\-and weighted MR imaging in vivo. 2. Synthesis of Fc-l>y iNCPs for magnetic resonance imagingFc-【)y NCPs were synthesized by1,lr-dicarboxyl ferrocene as building blocks andDy(【n) ions with highly paramagnetic properties as metallic nodes using ananoprecipitation method. Polyvinylpyrrolidone (PVP) was added in the synthesisprocedure to achieve water-solubility of the NCPs. The tbnned Fc-Dy NCPs werecharacterized by scanning electron microscopy (SEM), Courier transform infrared(FT-1R) spectroscopy and relaxivity measurements. It shows that the Fc-Dy NCPscould be丁2MR imaging contrast agent.