Screening For Tolerance Plants In Manganese Mining Tailing And Preliminary Research On Tolerance Mechanism

Most hyperaccumulation plants are herbaceous plants, these plants biomass is small, and easy to cause secondary pollution. In the face of such situation, the research take energy plant and timber forest and garden plant and hemp as research object, to screen Manganese tolerant plants and research mechanisms of mechanisms of manganese tolerance in plants. This study set four experimental treatment for Xiangtan Manganese slag (CK:100%slag+0.1Kg Phosphate fertilizer, Improved A:80%slag+15%peat soil+5%Organic bacterial manure+0.1Kg Phosphate fertilizer, Improved B:70%slag+25%peat soil+5%Organic bacterial manure+0.1Kg Phosphate fertilizer, Improved B:60%slag+35%peat soil+5%Organic bacterial manure+0.1Kg Phosphate fertilizer) to measure live plants growth situation biomass, plant height, heavy metal uptake and transfer characteristics and discuss the relationship between manganese tolerance in plants and chemical form of manganese and the subcellular distribution characteristics in plants that can offer the basis and underlying data for the ecological control and ecological use of lead-Manganese-polluted soil. The main results obtained from this study are summarized as follows:(1) The survival rate of plants in CK are low and only eight survived. This plants grow poor and small and growth was significantly suppressed. The surviving plants in CK are sapium sebiferum, nerium, paulownia, koelreuteria paniculata, ligustrum quihoui, ricinus, hibiscus cannabinus, corchorus capsularis. Through the matrix to improve plant survival rate was better than CK. The surviving plants in Improve A are sapium sebiferum, nerium, paulownia, cinnamomum camphora, koelreuteria paniculata, hibiscus syriacus, ligustrum quihoui, salix matsudana, ricinus, hibiscus cannabinus. The surviving plants in Improve B and C are sapium sebiferum, nerium, hibiscus syriacus, paulownia, cinnamomum camphora, cunninghamia lanceolata, koelreuteria paniculata, hibiscus syriacus, ligustrum quihoui, salix matsudana, ricinus, hibiscus cannabinus, corchorus capsularis.(2) From the perspective of biomass:different species difference is very big, but the trend of the same species are Improve C>Improve B> Improve A> CK, for example, the biomass of hibiscus cannabinus in Improve A, Improve B, Improve C respectively is as11.6,16.0and19.3times as CK. From the height:different species difference is very big and the same species under different processing plant height growth is difference. On the whole, the trend of the net increment of height are treated group> CK which plants and kenaf plant growth is most obvious. The net increment of height of salix matsudana in Improve A and B is87cm and93cm and hibiscus cannabinus in Improve A and C is118cm and109cm. The height of hibiscus cannabinus in Improve C is209cm at the end of experiment which is the highest of all the plants.(3) On the whole, different kinds of plants to the same differences between the uptake of the same heavy metal elements and the same kind of plant uptake of heavy metals are also different because of the kinds of heavy metals and substrate handling varies. The content of heavy metal element in plants is Mn, Pb, Zn, Cu from high to low and the trend of heavy metal absorption are Improve C>Improve B> Improve A> CK. The content of Mn, Zn, Cu in aboveground hibiscus cannabinus of Improve C respectively are1063.97mg,11.98mg and1.23mg which is the highest of all the plants. The content of Pb in aboveground paulownia of Improve C are6.96mg which is the highest of all the plants. The transfer coefficient is different between different species and the trend of transfer coefficient are treated group> CK. The highest transfer coefficient of Mn, Zn, Pb,Cu respectively is corchorus capsular is (Improve C,96.84%), sapium sebiferum (Improve A,91.40%), hibiscus cannabinus (Improve B,87.83%), sapium sebiferum (Improve A,95.53%).(4) The accumulation of Mn in the cell wall of the roots and stems and leaves might be one of the mechanisms of tolerance and hyperaccumulation of nerium and paulownia to Mn. The accumulation of Mn in the cell wall and the vacuole of the roots and stems and leaves might be one of the mechanisms of tolerance and hyperaccumulation of koelreuteria paniculata and sapium sebiferum to Mn.(5) The roots, stems and leaves of paulownia and nerium and koelreuteria paniculata and sapium sebiferum were extracted by different extracting solvents in lab, the results indicated that most Mn in paulownia were the speciation of protein chelating or inorganic salt or metal phosphate precipitation and most Mn in nerium were the speciation of protein chelating or phosphate precipitation state or soluble inorganic salt. Most Mn in the roots and stems of koelreuteria paniculata were the speciation of protein chelating or metal phosphate precipitation. Most Mn in the leaves of koelreuteria paniculata were the speciation of protein chelating or soluble inorganic salt. Most Mn in the leaves of sapium sebiferum were the speciation of protein chelating or soluble inorganic salt. Most Mn in the stems of sapium sebiferum were the speciation of protein chelating or metal phosphate precipitation. Most Mn in the roots of sapium sebiferum were the speciation of protein chelating. This results might be one of the mechanisms of tolerance and hyperaccumulation of this plants to Mn.