The Toxicological Effects Of Cadmium,Lead On Dugesia Japonica

Heavy metal pollution is becoming more and more serious, has been a serious threat to human survival and development, Cadmium and Lead are two main elements of heavy metal pollution, Planarian as water pollution biomarker is very sensitive to changes of its environment,the paper used planarians as material investigated the activity of antioxidant enzyme systems, the effects of SOD, CAT, GSH-PX, hsp70, CYP450, P53, wnt11gene expression, and studied the toxic effects on the nervous system of the planarian by immunohistochemical under the stress of Cadmium and Lead.1. The effects of antioxidant enzyme systems activity under the stress of Cadmium and LeadWhen planarian under different concentrations and time of cadmium, antioxidant enzyme activity changed obviously, the results showed that cadmium and lead can induced antioxidant enzyme activity, but high concentration can destroyed the structure of enzyme, and the long time stress activity gradually decline. The activity of different enzymes with different threshold, the metal cadmium and lead to the same enzyme activity influence mechanism is different.2. The effects of SOD, CAT, GSH-PX, hsp70, CYP450, P53, Wnt11gene expression under the stress of Cadmium and LeadThe experiment studied the expression change of coding genes SOD, CAT, GSH-PX and Hsp70.CYP450, P53, Wntll under stress of cadmium and lead.Amount of antioxidant gene expression is different from its activity, associated with degradation rate of protein and mRNA transcription. The expression of Hsp70, CYP450, P53,Wnt11changed obviously under stress of cadmium. Under normal physiological condition, Hsp70expression pattern, the expression level is very low, but high expression under stress of cadmium and lead to improve cell resistance. CYP450is an important enzyme, participate in a variety of exogenous substance metabolism, cadmium, lead stress can induce the expression quantity change; Cadmium, lead can damage DNA structure, repair and apoptosis mechanism can induce activation of P53;Wntll is closely related to the physiological processes can affect its expression.2. The toxic effects on the nervous system under the stress of Cadmium and LeadCadmium, lead can damage to a certain extent, the experiment can be obviously seen that nervous system of planarian been obvious fractured under the stress cadmium, lead with the increase of the concentration and time, planarian degree of the fracture of the nervous system is more and more serious.