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Toxicity And Effect On Anti-oxidant Enzymes Activity Of Tetra-bromodiphenyl Ether(BDE-47) In Marine Teleosts

Posted on:2015-11-13Degree:MasterType:Thesis
Country:ChinaCandidate:Y M ZhangFull Text:PDF
GTID:2181330431484013Subject:Ecology
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The marine environment pollution has become the focus of the world. There are a lot oftoxic substances get into the sea every year. Polybrominated diphenyl ethers (PBDEs), a kindof POPs, has been paid more attention in the word. As a brominated flame retardants, PBDEsis widely used in production and products of textile industry, rubber, plastic, electronicindustry, chemical industry, building materials, electric appliance and other fields. Becausethe chemical stability of PBDEs, it’s easy to be accumulated in organisms and biologicalamplification with food chain. The PBDEs has been detected in fish, birds, mammals andeven human body. It is reported that PBDEs has neurotoxic, reproduction toxicity,immunotoxicity and hepatotoxicity to organism. The highest concentration of PBDEs detectedin organisms is BDE-47, and the toxicity research is mainly in rodent. It is reported thatBDE-47has toxicity to marine microalgae. However, it has less report in marine fish toxicityresearch. The marine fish acts an important part in marine ecosystem. It is at a relatively hightrophic levels in food chain, and a most important food to human.In our experiment, we take the BDE-47in the highest levels of the body, as therepresentative of PBDEs homologue, by means of Karber method of calculation to researchthe acute toxicity test on Takifugu rubripes. Based on this study, we research the effect onantioxidant system of Takifugu rubripes liver treated with different concentrations of BDE-47(i.e., superoxide dismutase (SOD), Malondialdehyde (MDA), glutathione (GSH), glutathioneS-transferase enzyme (GST), glutathione peroxidase (GSH-Px), catalase (CAT)), in order toproviding a theoretic foundation for the study of BDE-47stressed and the mechanism ofoxidation toxicity of the liver in marine fish. In addition, we use the fin cell line ofEpinephelus fusco guttatus (GSB) to study the cytotoxicity effect of BDE-47(MTTmeasurement method, and a half inhibitory concentration (IC50)), and the effect on cellantioxidant enzymes (i.e., superoxide dismutase (SOD), glutathione S-transferase (GST),glutathione peroxidase (GSH-Px), Malondialdehyde (MDA)) in vitro. The aim to performthese in vitro and in vivo experiments is in order to establish a comprehensive, effective, rapid and sensitive BDE-47detection system, and provide favorable conditions of studying thetoxicity effect and the mechanism of BDE-47.The research carried out the acute toxicity test of BDE-47on Takifugu rubripes andfound that after24h,48h,96h, the LC50were70.01mg/L、18.83mg/L、9.44mg/L, separately,which determined a high toxicity on Takifugu rubripes according to the “New ChemicalSubstances Hazard Assessment Criteria (HJ/T154-2004)”.Sub-acute toxicity tests were madeto detect the content of MDA, GSH and the activity of SOD, GST, GSH-Px,CAT in liver ofTakifugu rubripes which was treated by different concentrations of low doses of BDE-47. Theresults showed that BED-47were able to cause significant changes of the activity and contentof biomarkers in liver of Takifugu rubripes with a dose-and time-dependent manner. Two setsof biomarkers can be created to combine with the detection of oxidative stress with BED-47to fish.The research determined the inhibitory ratio of BDE-47to GSB by MTT assay. Resultsshowed that5mg/L BDE-47was toxic to GSB, and the toxic effect was in a time and dosedependent manner. Based on the inhibitory ratio we calculated the48h-IC50of BDE-47toGSB was11.05mg/L.The research examined the activity of cytosolic SOD、GST、GSH-Px and the amount ofMDA in GSB after BDE-47treatment. Results showed that5mg/L BDE-47significantlytriggered the changes in all parameter we measured. The activity of SOD、GST、GSH-Px wasinversely proportional to the concentration of BDE-47, while the amount of MDA wasproportional. The activity of SOD、 GST、 GSH-Px first increased but decreased overtime,however the amount of MDA increased without attenuating. We can conclude from the resultsthat BDE-47can generate oxidative damage to marine fish, the change in SOD、 GST、GSH-Px activity and the amount of MDA can be utilized as an indicator of BDE-47toxicity.
Keywords/Search Tags:BDE-47, Takifugu rubripes, acute toxicity test, fin cells line of Epinephelus fuscoguttatus (GSB), cytotoxicity, anti-oxidant system
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