Determine The Content Of Plasticizer In Familiar Drinks By Fluorescent Probe Methodsand Its Application

Phthalate is the most common plasticizer; the flexibility is enhanced when added to the plastic, andmake it easy to process. It has the advantages of low volatility, good insulation, and plasticizing efficiency,high flame retardant and cheap price that is widely used in plastic, resin, rubber, cable, adhesive, cellulose,medical equipment and other products. But in recent years, plasticizer is abused seriously. It is addedillegally to food, drinks and food package, etc which causes food safety accidents that pose great threat tohuman, environmental pollution and series of serious consequences.The Ministry of Public Health hadaffirmed the plasticizer is the substance that cannot eaten and it is illegal to add into food. It is also bannedabusing as food additives. Therefore, it is urgent to develop methods for determination the content ofplasticizer.At present, the methods for plasticizer detection are chromatography, gas chromatography-massspectrometry, liquid chromatography-mass spectrometry, but these methods have the disadvantages ofexpensive, complicated in operation, complex pre-treatment and so on. Fluorescence spectrometry has theadvantages of less reagent, low cost, high sensitivity and fast detection. Therefore, it is necessary toestablishing new method for the detection of plasticizer in food by fluorescent probe.This paper is described from the following parts:We described the conception of plasticizer, the background and dynamic condition of plasticizerevent. We summarized the species, properties, application, mechanism of action, abuse, harm andcountermeasures. We also expounded fluorescence analysis method and its principle, and introduced somedetection methods for plasticizer and its application.We determined the plasticizer (DOP) in milk by acridine orange-Tweenn80systems, and determinedplasticizer phthalate of a certain brand of carbonated drinks; Fluorescent Pink-SDS system for thedetermination of DINP; Rhodamine B–OP system for the determination of plasticizers DPHP, and eosinY-CTMAB system for the determination of plasticizer DBP.We explored several fluorescent spectrometry methods for the determination of plasticizer:(1) InBritton-Robinson (B-R, pH=7) buffer solution, Tween-80can enhance the fluorescence intensity ofacridine orange, fluorescence intensity of the system increases sharply when added DOP, and the fluorescence intensity response shows a good linear relationship with the increasing of DOP, so weestablish a new method for the determination of plasticizer DOP.(2) In B-R buffer solution (pH=8), SDShas sensitivity effect on Fluorescent Pink, and fluorescence intensity of the system enhanced when addingDINP. With the increasing of DINP, response of ΔF and addition shows a good linear relationship, thus weprovide a fluorescence spectrometry method to determination DINP.(3) In Clark-Lubs buffer solution(pH=8) and OP solution, fluorescence intensity of the system can be enhanced when added DPHP, and thedegree of the enhancement shows good linear relationship with the content of DPHP, so we establish a newfluorescence method for the determination of DPHP.(4) In Michaelis buffer solution (pH=6.6), certainconcentration CTMAB, fluorescence intensity of eosin Y is enhanced when added DBP, and the responsevalue shows good linear relationship with the content of DBP, therefore, we establish a new method forthe detection of DBP.We studied the fluorescence spectra between fluorescent probe and plasticizer, fluorescent probe andsurfactant, plasticizer and surfactant. Besides, the mechanism of the methods that studied in this paper isalso discussed.