Investigation On The Electrochemical Detection Of Uric Acid Using Graphene Nanocomposite

Graphene is a new type of two-dimensional carbon nanomaterial, has receiving considerable interest due to its unique single atomic layer structure and excellent physical and chemical properties. Graphene exhibits large specific surface area, good biocompatibility and excellent mechanical, electrical, and thermal properties, thus making it a promising material in chemistry, biology, medicine, and physics. In this thesis, the preparation, functionalization and applications of graphene, were described, with emphasis placed on the construction of graphene-or its composites-based electrochemical biosensors.Graphene is capable of promoting electron transfer of the electrochemical reactions, and improve the sensitivity of the method and shorten the response time. Through functionalization, the stability, solubility, and dispersibility of graphene were further improved. Relying on the synergistic effect, graphene and thionine or ionic liquid, two kinds of nanocomposites of thionine-graphene oxide hybrid (T-GOs) and room temperature ionic liquid-graphene hybrid (IL-GNs) were created with ultrasonic assisted method and simple grinding, we synthesis thionine-graphene oxide hybrid (T-GOs) and room termperature ionic liquid-graphene hybrid (IL-GNs).Various techniques, such as UV-vis absorption spectroscopy, X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), atomic force microscopy (AFM) were utilized to characterize nanocomposites of the T-GOs and IL-GNs.Based on the above nanocomposites, two sensitive and stable biosensors for uric acid (UA) have been fabricated.These biosensors exhibite good performance for assay of UA in blood and urine samples. These robust and unique nanocomposite materials hold great promise on in biosensing, biomedical and microelectronic fields.