Investigation On Skin Permeation Of Transfersome As Transdermal Carrier

Transfersome is a flexible deformable nanoliposome. It is possible to overcome theskin barrier, that is wrapped with clear targeting drugs. It can be used as a good carrierto effectively control the drug release, improve bioavailability, and play the role ofsystemic treatment. Hyaluronic acid is a natural polymer material, with high moistureresistance. In the clinical field, skin medicament can be used as topicalpharmaceutical delivery carrier. In addition to its slow rate of drug release, It can alsopromote transdermal absorption of the drug and targeted to improve bioavailability.Prepared by vacuum rotary evaporation load HA-T NPs, by TEM analysis of HA-TNPs form a relatively uniform particle size, showing a multilayered spherical structure,having a good dispersibility. MEFs by hemolysis cytotoxicity experiments andexperiments on the blood compatibility and cytotoxicity of HA-T NPs, proved thatHA-T NPs has good biocompatibility.The DOX HCl package contained in HA-T NPs, using infrared spectroscopy andtransmission electron microscopy, observations confirm that the drug DOX-HA-TNPs successfully synthesized. Drug-loaded nanoparticles are spherical, its shape doesnot change significantly. Fluorescence microscopy DOX-HA-T NPs transdermalabsorption experiments and tissue sections showed that: DOX-HA-T NPs skinpenetration is higher than the amount of free DOX HCl solution and unloadedFITC-HA-GMS NMs nanoemulsion. It shows that HA-T NPs as drug carriers,significantly increases the efficiency of transdermal DOX, reaching the effect of thetreatment of the disease.DOX-FITC-HA-T NPs transdermal absorption experimental determines itsdistribution in various tissues inside. The results show that: the content of fluorescentnanoparticles in the body tissue of the distribution (from more to less, followed by):lymphoid tissue> spleen> heart> kidney> liver. Using HA-T NPs transdermal administration, the drug can be effectively reduced in various body tissue toxicity ofrats, accumulated in the lymphatic tissue targeting.MCF-7cells by fluorescence quantitative analysis of nanoparticle uptake andconfocal microscopy confirm: to DOX-FITC-HA-T NPs, FITC-HA-GMS NMsnanoemulsion and DOX-T NPs, MCF-7cells is in time-dependent inhibition ofuptake and concentration-dependent manner.In summary: HA-T NPs, as a novel nanoparticle drug delivery system, hasgained the feasibility of treating cancer and other diseases, after the packagecontaining the anticancer drug DOX HCl.