Toxic Effect Of TBBPA On Marine Microalgae

TBBPA,one of brominated flame retardants, is widely used and produced in theworld. Related research shows that TBBPA has a strong acute toxicity to aquaticorganisms. Marine microalgae as the primary producer in marine ecosystems plays animportant role in maintaining stability and balance of the aquatic ecosystem. Thecurrent toxicological studies related to TBBPA to marine microalgae is still quite rare,So this paper carried out a research to evaluate the impact of of TBBPA to the marineenvironment. Paper selected seven microalage in China Ocean University library forthe study, including Nannochloropsis oculata, Pyramimonas sp., Platymonassubcordiformis, Chaetoceros muelleri, Phaeodactylum tricornutum, Isochrysiszhanjiangensis, Tahitian Isochrysis galbana. Study is divided into three parts.1. The growth effect of TBBPA on the seven marine microalgaeThis study select seven marine microalgae, by analying the change of sevenmarine microalgae relative growth rate and inhibition rate under TBBPA treatment andusing Probability Unit-concentrationLogarithmic method, to study the growth effectof TBBPA on marine microalgae.this research obtains96h EC50of seven marinemicroalgae, The results showed that: The96h EC50of C. muelleri， N. oculata，Tahitian. I. galbana，I. zhanjiangensis，Pyramimonas sp. are respectively2.59mg·L-1,2.64mg·L-1,4.23mg·L-1,4.35mg·L-1,6.71mg·L-1, belong to highly toxic. The96hinhibition rate of P. tricornutum was22.84%，when exposed to the concentration of9.83mg·L-1of TBBPA. The96h inhibition rate of P. subcordiformis was only14.11%,when exposed to the concentration of14.6mg/L of TBBPA. C. muelleri and N.oculata are the most sensitive microalgae to TBBPA.2. The jiont toxicity of TBBPA and B[a]P on Isochrysis zhanjiangensis andPyramimonas sp.This paper designs the proper concentration of pollutants by the pre-experimental.Firstly, studying the effect of TBBPA, B[a]P and TBBPA+B[a]P onmarine microalgae growth.Then obtained96h EC50of microalgae in threecases.Finally, using Marking index method to evaluate joint toxicity of TBBPA andB[a]P on Isochrysis zhanjiangensis and Pyramimonas sp. The results show that:(1)TBBPA and B[a]P both belong to highly toxic to Isochrysis zhanjiangensis,whenTBBPA and B[a]P be mixed in the mixing ratio of500:3, joint toxicity index (AIvalue) is-1.23, less than0, joint toxicity is antagonism.(2) TBBPA and B[a]P bothbelong to highly toxic to Pyramimonas sp,when TBBPA and B[a]P be mixed in themixing ratio of4:1, joint toxicity index (AI value) is-2.03, less than0, joint toxicityis antagonism.3. The jiont toxicity of TBBPA and Cu2+on Isochrysis zhanjiangensis andPyramimonas sp.this paper designs the proper concentration of pollutants by thepre-experimental.Firstly, studying the effect of TBBPA, Cu2+and TBBPA+Cu2+onmarine microalgae growth.Then obtained96h EC50of microalgae in threecases.Finally, using Marking index method to evaluate joint toxicity of TBBPA andCu2+on Isochrysis zhanjiangensis and Pyramimonas sp. The results show that:(1)TBBPA and Cu2+both belong to highly toxic to Isochrysis zhanjiangensis,whenTBBPA and Cu2+be mixed in the mixing ratio of5:1, joint toxicity index (AI value)is-1.23, less than0, joint toxicity is antagonism.(2) Cu2+belong to middle toxic toPyramimonas sp,when TBBPA and Cu2+be mixed in the mixing ratio of5:2, jointtoxicity index (AI value) is-1.38, less than0, joint toxicity is antagonism.