Synthesis Of Fluorescent Nanoparticles And Research Of Fluorescence Test Strip For AOZ Residual Detection In Aquatic Products

Semiconductor nanocrystals (quantum dots, QDs) exhibit many unique optical properties such as high fluorescence intensity, narrow emission line, wide stimulation spectral line, continuously adjustable emission wave length along with particle size, and resistance to photobleaching, which can be exploited in optoelectronics, biomedicine and food safety detection. In this paper, we describe the synthesis of CdTe and CdTe@SiO2composites. The morphology and optical property were characterized. In addition, CdTe QDs were used as fluorescence probes for AOZ immunity-chromatography test strips. And these test strips were developed to detect the residual of AOZ in aquatic products.Water-soluble CdTe quantum dots, which were synthesized by the water phase synthesis method, quantum yield is30.8%. Polynuclear CdTe@SiO2composites were synthesized by inverse microemulsion polymerization, which exhibited spherical shape, uniform particle size, good dispersion and wonderful optical property. In the synthesis, the diameters of the CdTe@SiO2composites increased with increasing with increasing TEOS concentration, CdTe@SiO2composites with different diameters from60to70nm were synthesized by controlling the reaction conditions.CdTe QDs were labeled by AOZ antibody to synthesis immune-fluorescent probes. The coupling reaction could finish in25min and EDC·HC1, NHS were introduced300μL,200μL respectively. The optimal amount of AOZ antibody was600μL. The immune-fluorescent probes were applied in the fluorescent immunity-chromatography test strips and this strip detection limit of AOZ is10.0ng/g. Based on the above work, the fluorescent immunity-chromatography test strips were used to detect the residual of AOZ in aquatic product. The limite of detection of AOZ is10.0ng/g, false negative rate and false positive rate are less than5%, the cross-reactivity with SEM, AMOZ and AHD are less than1%. The fluorescent immunity-chromatography test stripscan improve the sensitivity and specificity of the detection and it has important practical value in the food safety detection.