The Absorption And Accumulation Of Triclocarban In Aquatic Organisms

Triclocarban (TCC), widely used antimicrobial agent, is one kind of pharmaceuticals andpersonal care products, which was widespread in the environment and had been detected inthe water, soil and organisms. TCC was regarded as environmental endocrine disruptor andpotential persistent organic pollutants. The pollution and its impact on ecological environmenthad attracted extensive attention. In this paper, analytical methods of trace TCC wereestablished to study the absorption, accumulation and release of TCC in aquatic plants, whichhad great significance for studying the environmental fate of TCC in aquatic ecosystems.A novel analytical method of trace TCC in aquatic plants had been developed usinghomogenized organic solvent extraction and silica solid phase extraction (SPE) clean upfollowed by rapid resolusion liquid chromatography equipped with an electrospray (ESI) andcoupled with tandem mass spectrometry (HPLC–ESI–MS/MS) analysis. Plants sample for2.00±0.02g (fresh weight) was dispersed with a high speed homogenizer and extracted twicewith20ml solvent (dichloromethane/acetone,4:1), then the extract was purified by silica solidphase extraction (SPE) and finally detected by liquid chromatography tandem massspectrometry. The linear range was between0.2–200ng/g with the correlation coefficientR2>0.999. The lowest detection limit (S/N=3) was0.05ng/g, while the limit of quantification(S/N=10) was0.17ng/g. Three concentration levels of1.0,10.0,50.0ng/g spiked recoverieswere91.80%–106.14%. The relative standard deviation of seven parallel samples was1.03%,while intra-and inter-day precision were less than9.25%and15.13%, so repeatability andprecision of the method could satisfy the experimental requirements. Sample matrix effectwas101.90%with better separation purification effect in line with the experimental analysisrequirements. The detection of aquatic plants found that the TCC concentration range was8.99–70.28ng/g, meaning the method worked well for the actual test.Through experimental simulation method, chrysopogon aciculatus, crown grass andgreen chrysanthemum were used to study TCC absorption and release in the three kinds ofaquatic plants, which showed it was easy to absorb TCC from water for aquatic plants,reaching equilibrium states after72hours. The enrichment coefficients of TCC forchrysopogon aciculatus, crown grass and green chrysanthemum were1474,493and1283.The study found that aquatic plants could absorb TCC by roots and leaves, and then TCCcould migrate among the root, stem and leaf. TCC concentrations in the root and leaf weresignificantly higher than in the stem.In addition, we also studied the distribution of TCC in the actual aquatic ecosystems (such as an artifical Lake) and the absorption and accumulation of TCC in the differentaquatic plants and fish, found that the enrichment factor was between844and4814in thedifferent aquatic plants, while it was3344in the barracuda (based on the amount of fat).