Research On Factors Affecting The Conversion Rate Of Liquid Fermentation Rice Bran Peptide And Significant Test And Analysis

Rice is an important human food grain; more than50%of the people in the world eatrice. Rice bran is a major by-product of the rice process. The rice production of the Chinaaccounts for about one-third of the corresponding rice production in the world. With thedevelopment of science research, researchers find that the rice bran peptide has a variety ofbiological applications, such as lowering blood pressure, reducing blood fat, immuneregulation, anti-oxidation and etc. Therefore, rice bran peptide is considered to be a highlypromising peptide. It is also valuable and important to extract rice bran peptide from rice.This study is mainly based on the technique of biological liquid fermentation usingorthogonal experimental design to explore the key factors which affect the conversion rate ofrice bran peptide. In addition, we explore the best extraction process, best combination ofvarious factors to extract rice bran peptide from rice. PH value is the most significant factorwhich effects the conversion of rice bran peptide, because it can affect the level of enzymehydrolysis and quality of rice bran peptide precipitation. Fermentation temperature affects thegrowth rate of the microbial flora. Fermentation time affects the conversion rate of growthduring the microbial flora. Solid-liquid ratio affects the conversion rate of rice bran peptide byinfluencing the growing space and density of microbial flora. The experiment results suggestthat rice bran peptide can be obtained most easily with fermentation temperature of30℃,fermentation time of96h and the pH value of6.5. In this circumstance, the conversion rate ofrice bran peptides can reach59.86%.To further analyze the impact of each independent factor for the final conversion rate ofrice bran peptide, we constructed the multivariate nonlinear regression model to predict theconversion rate of fermented rice bran peptide. Experimental results demonstrated that thepredictions quadratic polynomial model could exactly predict the conversion rate offermented rice bran peptide. This model will be helpful for the further analysis of rice branpeptide and supports for the production of rice bran peptide.