The Analysis Of Biochemical Composition Of Microalgae Culturedwith Seawater Of Oil Pollution And Preliminary Optimization Of Culture Conditions

With the increasing of human activities, the pollution of fossil fuel combustion and thedepletion of fossil resources, the humanity have more serious problems to solve. The worldare considering using a non-polluting, renewable energy instead of fossil fuels, biodiesel hasbeen concerned widely. Algae Chlorophyta (microalgae) for containing high fat, easy tolarge-scale cultivation, and the production per unit area is larger than soybeans and othercrops, etc., they’re considered as the latest or even the only biodiesel feedstock to achieve tosubstitute petroleum diesel. In this paper, we do the research about culturing the Chlorella sp.and Dunaliella salina with the seawater of oil pollution, and we do the determination ofprotein by using Coomassie brilliant blue, determination of total sugar content by Anthronecolorimetric, determination of fat content by Soxhlet extraction, as well as the determinationof the chlorophyll content. Explore the effects of light and inorganic salts on the Chlorella sp.and Dunaliella salina, it lays the foundation for the large-scale culturing marine microalgae ofpetroleum contained seawater.In this paper, culturing the Chlorella sp. and Dunaliella salina with the seawater of oilpollution, respectively, using the blood cell counting method, UV spectrophotometry,Coomassie brilliant blue and anthrone colorimetry to measure growth curve L, the chlorophylla content of intracellular and extracellular proteins and intracellular and extracellularpolysaccharide content of Chlorella sp. and Dunaliella salina cultured in the seawater with afinal concentration of0.5μ/mol petroleum continuous for21days. Studies show that theculture medium containing a low concentration of oil in the water, both Chlorella andDunaliella have good tolerance, but the Dunaliella salina has a better ability to grow, moresuitable for large-scale cultivation of sea water contained a low concentration of oil, it has ahigher value for development.The use of blood counts and UV spectrophotometry were measured the conditions of twomicroalgae cultured in0.5μmol/L in the standard oil by different light cycles, different lightintensities, adding different inorganic salts. The results show that the higher illuminationconditions and the longer illumination time can promote the two microalgae grow in theculture medium containing a low concentration of oil. The two microalgae can grow muchbetter with the conditions adding1500mg/L of sodium nitrate or1.8mmol/L of magnesiumsulfate or0.5mmol/L and1.5mmol/L of sodium molybdate. Using GC-MS to analyze affect of the easiest composition of the oil aromatic compounds-benzene homologues on the growth of Chlorella sp. and Dunaliella salina. The resultsshowed that0.5μmol/L of homologs of benzene in petroleum have a certain degree ofgrowth inhibition, the inhibition rates of toluene, ethylbenzene, m-xylene, p-xylene, o-xyleneon Chlorella sp. were58.63%,58.99%,60.07%,64.03%,65.47%; the inhibition rates ofDunaliella salina were48.47%,49.08%,64.63%,62.50%,59.75%. Meanwhile the utilizationof the two microalgae oil for homologues of benzene are low, about1-2%.