Studies On The Interaction Mechanisms Between Silver Nanoparticles And Proteins

People focus on nanomaterials for their unique properties, especially for their bright application inenvironmental monitoring, biomedical, industrial catalysis and other fields. Among them, silvernanoparticles with excellent photoelectric properties are of intriguing interest. As one of most popularsurface enhancement Raman scattering active substrates, silver nanoparticles have been used to obtainmillion fold enhancement in Raman scattering, which highly improved the detection sensitivity. At thesame time, silver ions have been shown to be highly toxic. A great potential of silver nanoparticles is thatthey can incorporated into the field of life science research. Proteins that paly an important role in lifeinteraction with silver nanoparticles as research in this paper, which is of great theoretical and practicalsignificance to explore the effects of nanoparticles on living organisms and the mechanism.Protein play an important role in the process of life and researching about life science. Life activitiesrely on the protein. So when we desire to have further understanding about the interaction of nanomaterialsand biosystem, protein is chosen as the first choice. The study about the interaction of nonmaterial andprotein is not only will improve the exploration of namomaterial in biological system, but also HSAsignificance on system research about nonmaterials with biological mechanism.The interaction and correlation effect about silver nanoparticles with goblin, such as bovine serum,lysozyme and human serum albumin, HSA been investigated by different spectral method. Thedecrease and red shift of416nm ultraviolet absorption peak of Ag NPs show that the three kinds of goblincan be adsorbed on the surface silver nanoparticles. The Stern-Volmer and van der Hoff equation suggestthat the mechanism may be a static quenching and the binding constants are between10-9～10-10L mol-1.The main chemical of the quenching is the formation of silver nanoparticle-proteins aggregates. Thesynchronous fluorescence and UV spectrum reveals that the microenvironments around amino acid residueswere destroyed by silver nanoparticles, to increase the hydrophobic medium and change the conformationof protein. The correlation effect about silver nanoparticles with globin is double hysteresis effect, which iscoating the silver nanoparticle by protein molecules and the conformation changes of proteins. Theadsorption of three kinds of goblin on Ag NPs was analyzed by Langmuir and Freundlich models, turning out that the equilibrium adsorption data fitted well with Freundlich model. Using the pseudo-first-order andpseudo-second-order kinetic equations study the equilibrium adsorption data. The results suggested thatpseudo-second-order kinetic equation would better be suited to the adsorption kinetics. These researchesprovided valuable reference experimental basis on the application of silver nanoparticle in organisms.