Different Treatments On Storage Of Pleurotus Eryngii Influence Of Process Quality And Relative Enzyme Activities

[Objective] To study the effects of different treatment conditions on the physiological and biochemical changes of Pleurotus eryngii, and ultimately to determine the most appropriate storage conditions of Pleurotus eryngii, then we want to establish the HACCP plan for postharvest cold-chain logistics of Pleurotus eryngii.[Methods] Packing of Pleurotus eryngii in different vacuum (0MPa、0.04MPa、0.06MPa、0.08MPa), different storage temperature (0℃、2℃、4℃、6℃、8℃) and different thickness of packaging (0.03mm、0.05mm、0.07mm). For every5days, we measured the color, hardness, chewiness, elasticity, cohesion, respiration intensity of Pleurotus eryngii, and determined the contents of CAT, SOD, PPO and MDA at the same time which lasted for30days in all. After choosing the optimal vacuum, storage temperature and packaging thickness, then we determined the most suitable conditions by three-level and three-factor orthogonal test. In the test, the color of the Pleurotus eryngii was measured using the colorimeter. The hardness, chewiness, elasticity and cohesion of the Pleurotus eryngii were determined using the texture analyzer. The respiration intensity was measured using standing for alkali absorb method. The contents of CAT, SOD and MDA were studied using the related kits. PPO was determined by spectrophotometer. By hazards analysis of postharvest cold-chain logistics process of Pleurotus eryngii, we identified the critical control points and key constraints and then developed the HACCP plan for postharvest cold-chain logistics of Pleurotus eryngii.[Results](1) When the other storage conditions was fixed, the color, PPO and content of MDA of Pleurotus eryngii in0.06MPa processing group were less than those in other groups, and the differences were significantly (P<0.05or P<0.01). The elastic, chewiness, CAT and SOD were greater than those in other groups, and the differences were significantly (P<0.05or P<0.01). The hardness and cohesion of Pleurotus eryngii in0.08MPa processing group were greater than those in other groups, and the differences were also significantly (P<0.05or P<0.01).(2) When the other storage conditions were remained unchanged, the color, respiration intensity, PPO, and the content of MDA of Pleurotus eryngii which were stored under2℃were less than those of Pleurotus eryngii in other groups, and the differences were significantly (P<0.05or P<0.01), but compared with the Pleurotus eryngii stored at4℃, these differences were not significantly. The hardness, elasticity, cohesion and chewiness of Pleurotus eryngii which were stored under2℃were greater than those of Pleurotus eryngii in other groups, and the differences were significantly (P<0.05or P<0.01), but compared with the Pleurotus eryngii stored at4℃, the differences were not significantly Under4℃, the CAT and SOD of Pleurotus eryngii were greater than those of Pleurotus eryngii stored at other temperatures, the differences were significantly (P<0.05or P<0.01), but compared with the Pleurotus eryngii stored at2℃, the differences were not significantly.(3) When the other storage conditions were stayed the same, the hardness, cohesion, elasticity, chewiness, respiration intensity, SOD, CAT of Pleurotus eryngii packed with thickness of7mm were greater than those in other groups, and the differences were significantly (P<0.05or P<0.01). The color, PPO and MDA of Pleurotus eryngii packed with thickness of7mm were less than those in other groups, the differences were also significantly (P<0.05or P<0.01).[Conclusion] The ideal storage conditions of Pleurotus eryngii:vacuum degree is0.06Mpa, temperature is2℃～4℃, and package thickness is7mm. Through the hazard analysis of postharvest cold-chain logistics of Pleurotus eryngii, we determined the acceptance and storage as the critical control points. Based on national standards and previous experimental results, we established the critical limits and developed the HACCP plan for postharvest cold-chain logistics of Pleurotus eryngii.