Transcriptome Analyses Of Different Tobacco Leave

Tobacco (Nicotiana tabacum) are major agronomic crop and hobby consumers，it iswidely grown in the world. The position of tobacco is compelling in term of finance andeconomic policies in many countries. In all plants, the tobacco leaves which as harvest cropsare incapable of edibility. After the special modulation processing, the smoke from leavesburning is the way of consumption., this feature let us to pay more attention to study tobacco.And tobacco is not only an important economic crop, but also a model organism of plantsfor science research. Next generation sequencing technology have many advantages becauseit generated large-scale sequencese more costeffective, highly sensitive and accurate. It is apowerful and quantitative approach has revolutionized promoted the development of denovo transcriptome sequencing at high resolution.This study on transcriptome sequencingof four tobacco widely grown domestically, Zhongyan100, Yunyan202, Yunyan87, andYuyan6, with next generation sequencing technology and splicing by different software,function annotation, and analyses on classification and metabolic pathways of the sequenceddata:1) Splicing the mixed sequences of four breeds with Trinity and obtaining62M data. Weannotated unigene in Ns and Swiss-protein, classified by COG and GO, and made analyseson KEGG metabolic pathways. Then we made local BlastX with obtained unigene data andthe database of Nr and Swiss-protein to achieve the best annotation. We made blast analyseson the obtained unigene and acquired COG function classification of tobacco expressedgenes, of which the22180sequences that gained homogeneous information were classifiedinto24categories and underwent comprehensive functional prediction.28013unigenes thatobtained GO annotation information were classified into3categories and subdivided into51subclasses.4813unigene could map into227metabolic pathways and obtained relatedinformation.45557coding sequences had54WRKY transcription factor which played a roleof response function in many biological processes such as environmental stress, biologicalstress and so on, which was consistent with the existing research.2) We spliced Zhongyan100and Yunyan87respectively, focused more on theirdifferentially expressed genes. Zhongyan100and Yunnan87got38891and67625unigenerespectively.3209unigene with significant differences in expression was filtered.2153differential expression gene was highly expressed in Zhongyan100.1056differentialexpression gene was highly expressed in Yunyan87. We found that12family members ofAP2/ERP were active in Yunyan87via GO. Linolenic acid-Jasmonic acid metabolic pathway showed significant difference in the two breeds by KEGG. The coding genes of14necessary enzyme were completely highly expressed in Zhongyan100.3) Based on the newly released genomes of Nicotiana tabacum L. and Nicotiana rustica L.,we map spliced the four breeds and compared the spliced results of Cufflinks、Trinity、Abyss.On account of the reference sequences, the result of Cufflinks was best and we determined68TIR-NBS-LRR gene.