Isolation Of Phenanthrene-Degrading Microorganisms The Biochemical Conversion Of Fharmaceutical Intermediates

PAHs are environmental carcinogens.The ratio of PAHs was beyond30%in environmental carcinogens. PAHs have mutagenecity and carcinogenicity and can enrich through the food chain. Bioremediation is an Efficient clean approach for the cleanup of sites contaminated with polycyclic aromatic hydrocarbons (PAHs),which has got extensive research.Bacillus.SW with degradation ability of PAHs and organophosphorus pesticide is isolated from contaminated sediments in the river. Bacillus.SW can degrade OP-DDT, PP-DDE, PP-DDD, PP-DDT, methyl parathion and parathion. The objective of this study was to research phenanthrene degradation mechanism of Bacillus.SW, degradation system optimization and pharmaceutical intermediates catalytic conversion.GC-MS analysis of phenanthrene degradation of Bacillus.SW confirmed biodegradation by detection of intermediates. The results indicated that Bacillus.SW can degrade phenanthrene through two pathways, which are3,4carbon ring opening and9,10a carbon ring opening. A8%increase is observed by changing membrane permeability. The biodegradation data of phenanthrene increases about25%,18%and43.5%degradation at the addition of maltose, polysorbate80and glycerin.Introduction of a group of natural microbial strains pseudomonas with salicylic acid can enhance the rate of degradation. With a proportion of1:1and8days addtion time interval,the degradation of phenanthrene increases46.5%. A35%increase is observed as a result of cell fusion. Bacillus. SW is also used for catalytic conversion of pharmaceutical intermediates.The results indicate that Bacillus. SW has the ability of catalytlo hydroxylation. It can transform salicylic acid into gentian acid.A reasonable enzyme blockers SDS is found to restrict the reaction to the hydroxyl steps.