Study Of Biohydrogen Production By Chlamydomonas Reinhardtii In Monosodium Glutamate Wastewater

Hydrogen is an ideal energy source that it would be the substitute of the fossil fuels to solute the energy and environment problem. Monosodium glutamate wastewater is difficult to treat and in a large amount. Therefore, combining the Monosodium glutamate wastewater treating and biohydrogen production will obtain a great of advantages in economy, entironment and the society.In order to produce hydrogen by Chlamydomonas reinhardtii in monosodium glutamate wastewater. The effect of monosodium glutamate wastewater to C. reinhardtii growth and hydrogen production was studied.TAP medium was the best for C. reinhardtii growth. The best carbon source is lml/L acetic acid and nitrogen source is0.4g/L ammonium chloride. The cell density could achieved to6.51×106cell/ml when C. reinhardtii grown in such medium.Adding glucose, glutamic acid or urea to TAP medium can increase C. reinhardtii cell density and chlorophyll content.The best concentration of glucose, glutamic acid and urea are0.2g/L、0.3g/L、0.1g/L respectively. Ammonium Sulphate could be used as nitrogen source and facilitated the growth of C. reinhardtii.The best ammonium sulphate was0.5g/LHigher hydrogen evolution could achieve by Sulfur deprivation in TAP medium (TAP-S). Hydrogen accumulation was1627μl/bottle in6days, which was8times than it in Sulfur repletion TAP medium. The hydrogen evolution was always better when sulphate concentration was lower than75μmol/LAdding glucose, glutamic acid or urea to TAP-S medium can increase the hydrogen production. The best concentration of glucose, glutamic acid and urea are0.2g/L、0.3g/L、0.1g/L respectively. Most important, the glucose promoted hydrogen evolution greatly. It was2.7times than the hydrogen accumulation in TAP-S medium.After pretreatment and dilution, the complex wastewater, ion-exchanged wastewater and isoelectric wastewater could be used for C. reinhardtii incubation. All the wastewater diluted by water in8times is the most appropriate procedure. After C. reinhardtii cultured, COD was changed to43.14%、48.3%、57.12%respectively. What is more, as medium of C. reinhardtii, the isoelectric wastewater was the best of all the three type wastewater. However, hydrogen production in such wastewater was less than it in TAP medium. Hydrogen production was1.29μl/ml and0.96μl/ml in complex wastewater and isoelectric wastewater respectively.