Study On Preparation And Properties Of Antimicrobial Drug Loaded Wound Dressing

Ageing population causes cutaneous wounds represent a major health careburden in china. However, limited information exists regarding the epidemiologicchanges associated with recent social and economic development. Whereby, Bacterialinfection is one of the significant factors affecting wound healing process，of whichwound exudate, containing the inflammatory cytokines, proteases and free radicalswill slow wound healing. Research and development of novel antimicrobial dressingsbecause of its good resistance to infection, bacteria and promote the healing, whichhave important implications for treating surgery wound infection, that is the inevitabletrend of the development of wound dressings. New antibacterial dressing has a goodanti-infection, bacterial resistance, promote healing and other effects. Its research anddevelopment undoubtedly brought heal and alleviate the suffering hope for thepatients with chronic wounds. Therefore, the future of antimicrobial dressings in theworld used and the prospect of market will be more widely.Natural polymer chitosan (CS) has broad-spectrum antibacterial activity andpowerful hemostatic function, non-harmful degradation products, has good biologicalcompatibility, bioactivity and bio-degradability, which can effectively promote woundhealing and tissue repair and regeneration in the field of biomedical dressing hasbroad application prospects. Polyethylene oxide (PEO) as a biological material whichhas good biocompatibility, low toxicity, non-irritating. Which have good spinnability,PEO and CS blended in order to improve CS spinnability.Nanofibers prepared by electrospinning technique with a small diameter, largesurface area, the structural characteristics of high porosity, while the ciprofloxacin andmoxifloxacin hydrochloride is loaded into the CS/PEO nanofibers, the use of chitosanand synergistic antibacterial effect of antibacterial drugs, can effectively improve theantimicrobial properties of the materials. The antibacterial fibrous mat has potentialprospects in the biomedical dressings field. CS/PEO90/10was chosen as raw material,90%aqueous acetic acid as solvent,ciprofloxacin and moxifloxacin hydrochloride as model drugs, to prepare CS/PEOand Drug-CS/PEO blend spinning solution separately, then electrospinning wascarried out. Electrospun fiber morphology was observed by scanning electronmicroscopy (SEM) and diameter distribution. The influence of solution parameters(spinning solution concentration) and the spinning process parameters (voltage,distance, humidity, feed rate) on fiber morphology were systematically investigated todetermine the optimal blend spinning system and process parameters were as follows:the concentration of spinning solution at5wt%, feed rate at0.8mL/h, distance at20cm,and spinning voltage of10kV.The CS/PEO90/10electrospun fibrous mats were cross-linked by glutaraldehyde(GA) vapor. Fibers morphology characterization, mechanical property, with waterabsorption and dissolution performance were applied to observe the influence ofelectrospun fiber membrane structure and performance after cross-linking differenttime. It is concluded that the optimum crosslinking time by8h. The compostion andstructure of electrospun fiber mat，before and after crosslinking and drug loaded werecharacterized by infrared spectroscopy (FT-IR) and X-ray diffraction (XRD), and theresults showed the new C=N bond was formed between CS and PEO moleculars aftercrosslinked, which produced a strong interaction and reorganization of the originalcrystal structure. The introduction of small molecule drug didn’t have an impact onthe morphology and structure of the electrospun membrance.In addition, this paper studied the drug release property of fibers membrane inhuman body fluids, as well as antibacterial property against (staphylococcus aureusand Escherichia coli) of CS/PEO nanofibers membranes with or without drugs.Cytotoxicity property of drug loaded membrane before and after crosslinked. Theresults showed that, drug in the fiber membrance was slowly released by dissolvingmechanism, overcoming the burst release of ordinary medical dressing used in intialstage. The cumulative release rate of seven-day up to86%,(5%CipHCl),89%(10%CipHCl),75%(5%Moxi) and77%(10%Moxi), respectively. With or without drugof fiber membranes all have antibacterial properties, with increasing drug loading andantibacterial properties also correspondingly increase, which indicated that theintroduction of antimicrobial drug makes antibacterial properties of nanofibermembrane reinforced. Cytotoxicity test results showed that, whether CS/PEOelectrospun fiber membrane crosslinking and drug loaded or not, cell activity was reached more than85%, shows that after crosslinking and drug loaded of fibermembrane have low toxicity to cells.Finally, this paper for drug loaded electrospun mats as functional layer, alginicacid non-woven fibers as adsorption layer, polyester film as a support layer formingantibacterial medical dressings, compared the properties with domestic and foreignAntibacterial medical dressingsThe results show that the homemade medicine antimicrobial wound dressingsantibacterial properties, fluid absorption and retention were superior to commercialantibacterial medical dressings.