Synthesis And Research Of Organic-inorganic Hybrid Nanomaterials

“Nano-composite Material” contains two or more substances combined together fromnanoscale to form a new composite material with some special functions. The newly formedcomposites exhibit some special characteristics and endow them many new excellent properties.Organic-inorganic hybrid nano materials have excellent characteristics because of takingadvantages of organic materials, inorganic materials and nano materials. Applications of thesematerials in the fields of mechanics, thermals, optics, electronics, biological and environmentalprotection have been reported. These new type materials will be used widely in high technologyfields in the future. Polystyrene microspheres also have a wide range of applications in these areas.For the special physical chemistry properties and practical applications, especially the applicationin biological separation and detection, the research of polystyrene-based andpolystyrene-inorganic nanometer compound material are eliciting widespread interest. In thispaper, in order to promote the application of polystyrene, the synthesis of organic-inorganic hybridnano materials and magnetic nanocomposites were carried out systematically. The details areshowed in the following parts.First of all, in-situ polymerization method was used to synthesize uniform Fe3O4/PVPnanoparticles and the effects of concentrations of Fe3O4, the reaction time and temperature on theirproperties were explored. The products were characterized by transmission electron microscopy(TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and VibratingSample Magnetometer (VSM). TEM and VSM images showed that superparamagnetic Fe3O4 /PVP nanoparticles with a size of18.6nm and FT-IR proved that Fe3O4/PVP nanoparticles weresuccessfully prepared.Second, the magnetic nanoparticles and styrene were emulsified separately and the magneticmicrospheres of carboxylic poly-styrene (St) were synthesized by the modified emulsion-polymerization. Meanwhile, effects of the monomer ratio ([St]/[MMA]), concentrations of emulsifier andFe3O4nanoparticles on their properties were explored. Then, the uniform polystyrene/polysilsesquioxane (PMPTS) nanoparticles were synthesized by emulsion-polymerization and the effects ofthe volume ratio ([PMPTS]/[St]) was explored. The results demonstrated the Fe3O4/polystyrene/MMA nanocomposite microspheres having preferably micromorphology and a mean size of160mm with strong magnetism were obtained with the ratio of ([St]/[MMA]) at20within20h. Dueto the surface of MMA with carboxyl, it can improve the hydrophilicity on the surface ofpolystyrene magnetic microspheres.Finally, the UV-visible absorbance measurement for antibody binding with antigen showedthat a mouse anti human myoglobin antibody (MYO) was successfully connected on surfaces ofthe composite microspheres, laying a foundation for the realization of the in vitro diagnosticapplication. The result showed that the optimal concentration of MYO antibodies is1.3mg/ml.