Study On Flavonoid Contents And Pigments Of Chinese Jujube

Jujube is the fruit of rhamnaceae jujube plant which is originating in China; it isChina’s best traditional food as well as the medicine. There is rich vitamins, minerals andother nutrients as well as cyclic adenosine monophosphate, triterpene acids and otherbioactive substances in jujube, studies had shown that jujube had the functions ofantioxidant, anti-aging, cardiovascular disease curing, blood pressure lowering and otherhealth functions. In this paper, took red jujube as the raw material,used enzyme auxiliaryultrasonic method, optimized the extracting conditions through the ternary quadraticregression orthogonal rotary design and antioxidant extracts were evaluated.Jujubeflavonoids has carried by HPLC-MS on the qualitative analysis.HPLC method for threekinds of flavonoids content in jujube were determined; Optimization of fructus corni redpigment extraction conditions and macroporous resin, the RP-HPLC purification, so as toprovide theoretical basis for the comprehensive processing and utilization of red jujube.The main results were as follows:Determined flavonoids extraction conditions of the red jujube, the choice of enzymeassisted ultrasonic method was the best method, studied the influences of time, temperature,pH, solid-liquid ratio, ultrasonic power, the amount of enzyme, extraction concentration onthe total flavonoids extraction rate. Determine the optimum extraction conditions throughsingle factor and ternary quadratic rotation regression orthogonal test were: the extractionsolvent concentration was60%, time was50min, ultrasonic power was180W, pH was4,temperature was45℃, enzyme dosage was0.6%, liquid ratio was1:25, in this conditionthe total flavonoids extraction was0.546%, DPPH clearance rate was41.8%.LC-MS technique using a qualitative analysis of myricetin, rutin, epicatechin jujubecontains. The use of HPLC technique, a quantitative determination of the three flavonoids,liquid phase process of three flavonoids: HYPERSIR C18BDS (5μm×250mm×4.6mm); mobile phase was the mobile phase A methanol, mobile phase was the mobile phaseB0.1%formic acid water; flow rate was0.8mL/min; the detection wavelength was:270nm,370nm; column temperature was30℃; injection volume was10μL. Use gradientelution method, the test results showed that: jujube contains0.0084mg/g Myricetin;0.0326mg/g rutin; epicatechin pigment content was0.0409mg/g.Measurement wavelength of the jujube peel was510nm; the best extraction solventfor the jujube red pigment extraction method was sodium hydroxide solution by extracting temperature, time, solid-liquid ratio, extraction agent concentration to have effect on jujubepeel red pigment extraction rate. The optimum extraction conditions through single factorand ternary quadratic rotation regression orthogonal test were: water bath temperature was70℃, bath time was4.0h, solid-liquid ratio was1:25, extraction agent concentration was2%. The significant order of each factor was: water bath temperature> extraction agentconcentration> bath time> solid-liquid ratio. Crude extract pigment was further purified bymacroporous resin, the results showed that: macroporous resin AB-8had good adsorption,desorption effect for pigment, and the optimal test conditions were: pigment concentrationwas0.8mg/mL, adsorbed for120min at room temperature, after completion of the resinrinsed, use eluant (50%ethanol) for desorption at room temperature.The flavonoids extraction, separation, characterization for the red jujube and the redpigment extraction and purification for the peel of the red jujube were studied in order toprovide process parameters and theoretical basis for the red jujube’s processing andutilization. The main results were as follows: through the study, found that red jujubecontains rich flavonoids, took rutin as the standard, and established the approach tomeasure the total flavonoids extraction rate in red jujube with ultravioletspectrophotometry.Conduct RP-HPLC method to prepare separation after the resin purification of thejujube peel red pigment. Studied the factors of the type of preparative chromatographyeluent, the ratio, the flow rate and other factors. Obtained preparation conditions:chromatographic column C18column (10μm,20mm×300mm); flow rate is10mL/min;mobile phase methanol: water (50:50); detection wavelength was310nm; injectionvolume was0.2mL. Pure product of more than95%purity was obtained; conductedanalysis by liquid chromatography-mass spectrometry for the purity, the measuredmolecular weight was341.1.