Analysis Of Bacterial Diversity In Activated Sludge During Ecological Stabilization

The sewage treatment system as a special microbial flora, the symbiotic relationship between microbes and their role in wastewater treatment has been more and more attention on the activated sludge, it is very necessary to us to have some research to the microbes and their diversity.In order to explore the bacterial diversity in activated sludge during ecological stabilization, the PCR-DGGE technique was utilized to investigate the variation of the bacterial community structure at different time and area. Statistics showed that the flora features of samples taken from different location at the same time were quite similar, while there was some difference between samples taken at different time. Shannon index and Pielou index were climbing from March to September, indicating the increase of biomass and degree of homogeneity. The majority of the stains were confirmed as actinomyces or mycetozoan according to DGGE strips sequncing and Phylogenetic Analysis. The increase in the number of Beijerinckiaceae and Rhodococcus favors the degradation of organic matter. The decrease of pathogenic bacteria like Pseudonocardia indicates the stabilization was effective.