| The implementation of integrated management in Liaohe reserve is aimed atimproving and restoring the Liaohe water environment and water ecology. Due to theacceptance of surrounding city sewage and industrial waste water, when thetributaries of Liaohe feed into the main stream, it becomes an important source of themain pollutants. By combining the two, restoring wetland purification functions andconstructing engineering, in order to reduce tributaries pollution load and enhancewater self-purification ability. This paper detected the water quality in Liaohe reserve.The research focused on the composition, origin and migration in soluble organicmatter (DOM) that distributed in the main stream， tributaries and estuarine wetland,and the microbial diversity and structure analysis of different plants rhizosphere inQixing wetlands, further reveal the environmental behavior characteristics ofsoluble organic matter in Liaohe reserve and microbial effect on different plantgrowth environment. It can provide the basis data of water ecological restoration inLiaohe reserve.On the basis of monitoring water quality indicators of Liaohe River, the source,structure and humification degree of dissolved organic matter (DOM) in sedimentpore water were investigated by combined with flourescence regional integration (FRI)and three-dimensional excitation-emission matrix fluorescenc spectroscopy(3DEEM).The indicator of the fluorescence index of water situation were alsoexplored. The results showed that the water quality indicator of Liaohe River wasbad.The fluorescence index f450/500，ranged from1.53to1.92, average value at1.71,indicated that the humus among Liaohe reserve DOM was the main biological source.The variation trend of organic contents in these five fluorescent areas werecharacterized by the results of FRI. Fluorescence organic matters, such as protein-likedegradable organics, accounted for44.15%～66.50%, was the main pollutant.Humification index r(B, D)was1.70±0.094in the trunk stream,1.726±0.231intributary, and1.662±0.138in estuary wetland. The Pearson correlation analysisresults showed that the fluorescence index could indicate of DO and NH4+-N.Therefore, the combination of3DEEM and FRI method could be used to represent therelative concentration of DOM effectively, and the fluorescence index could be used to indicate the changes of water quality.The clone libraries indicated that Proteobacteria was the main group inrhizosphere microorganisms of reeds, calamus and typha. And most microorganismsbelonged to Betaproteobacteria. The second group was Acidobacteria. Bacteroidetes,Firmicutes, Nitrospirae and Verrucomicrobia accounted for various proportion indifferent plants. The microbial populations had a close relationship with the plantgrowth and wetland purification function. |
