Synthesis Of Magnetic Nanomaterial And Its Application On Separation And Analysis Of Proteins

In this paper, we combined immobilized metal ion affinity chromatography and molecular imprinting technology, respectively, with the method of distillation precipitation polymerization, finally synthesized two kinds of functional magnetic nanomaterials. Then we applied these materials into separation of protein molecules.This paper was divided into three sections, including introduction, facile synthesis of a Ni(Ⅱ)-immobilized core-shell magnetic nanocomposite as an efficient affinity adsorbent for the depletion of abundant proteins from bovine blood and the synthesis of magnetic lysozyme-imprinted polymers by means of distillation precipitation polymerization for selective protein enrichment.In chapter one, immobilized metal ion affinity chromatography and molecular imprinting technology were mainly summarized. Also we focused on magnetic nanomaterials in the field of separation and analysis.In chapter two, Firstly, Fe3O4magnetic nanoparticles (MNPs) were synthesized through a solvothermal method and then were conveniently surface-modified with3-(methacryloyloxy) propyltrimethoxylsilane as anchor molecules to donate vinyl groups. Next a high density poly(4-vinylbenzylchloride) shell was synthesized on the surface of silica-coated Fe3O4MNPs via distillation precipitation polymerization. After the pVBC shell reacted with iminodiacetic acid in alkaline aqueous solution, the magnetite was charged with Ni2+to form the final product Ni(Ⅱ)-IDA functionalized hybrid nanoparticles. Subsequently, these affinity nanomaterials were applied into separation of bovine hemoglobin and the results indicated that they could quickly eliminate high abundance protein from bovine blood sample.In chapter three, Fe3O4magnetic nanoparticles were synthesized through a solvothermal method and then were directly vinyl-functionalized by sol-gel. The molecular imprinted shell was coated onto the surface by the copolymerization of functional monomer acrylamide, cross-linking agent N,N’-methylenebisacrylamide, the initiator azodiisobutyronitrile and template lysozyme through the method of distillation precipitation polymerization. Finally, the Magnetic Lysozyme-Imprinted Polymers were obtained after the elution of template protein. The adsorption experiments showed these nanomaterials possess a high adsorption capacity and good selectivity. These molecular imprinted nanomaterials were applied into separation of template and other proteins. The results exhibited they had a good selectivity towards the template and enabled fine separation of lysozyme from egg white samples.