A Preliminary Study On The Residual Status Of Phenolic Endocrine Disruptors And The Microbial Degradation In The Offshore Typical Area

Alkyl phenols are a class of typical endocrine disruptors, distributed in the environment Widely, have the significant biological accumulation and the female hormone effect, can pass through the food chain, endanger human health Ultimately. Environmental distribution and behavior of Alkyl phenols has become a hot research field of environmental science.In the study, six kinds of phenolic endocrine disruptors as research subjects, analysis method of this kind of compounds were built in various environmental media, and carry on an investigation into the distribution of phenolic endocrine disruptors in the coastal waters of the northern Yellow Sea. The experimental results were as follows:Established analysis method of detecting six kinds of phenolic endocrine disruptors in environmental samples using HPLC-MS/MS. Using dichloromethane as extraction solvent, water samples were extracted using liquid-liquid extraction method, the spiked recoveries were in the range of69～90%, the relative standard deviation was less than10%. Using accelerated solvent extraction for biological samples and sediments, the spiked recoveries were in the range of67～89%and61～85%, the relative standard deviation was less than10%. Using amino solid phase extraction column, the sample was extracted and purified by dichloromethane-n-hexane and methanol successively, the problem of the high fat and protein content and extraction, purification process complex were solved basically. Mass spectrometry with negative ion scan mode. Under the selected chromatographic conditions, each phenolic endocrine disruptor could achieved baseline separation, in the range of0～100μg/L, the linear relationship was well, the variation coefficient was0.9976～0.9990. In water, the method detection limit of nonylphenol, octylphenol, bisphenol A, tert-octylphenol was0.5ng/L; The method detection limit of2,4-dichlorophenol and p-tert-butyl-phenol was1.0ng/L. In biological samples, the method detection limit of nonylphenol, octylphenol, bisphenol A, tert-octylphenol was1.2ng/g; The method detection limit of2,4dichlorophenol and p-tert-butyl-phenol was1.0ng/g. In sediments, the method detection limit of nonylphenol, octylphenol was0.50ng/g; The method detection limit of bisphenol A tert-octylphenol,2,4-dichlorophenol and p-tert-butyl-phenol was0.8ng/g. The method was high sensitivity, strong anti-interference ability, high accuracy and precision, high recovery rate and stable. This method of the separation and analysis of phenolic endocrine disruptors in marine biological tissue laid a certain foundation.Residues of phenolic endocrine disruptors in the coastal waters of northern the Yellow Sea were tested through the established method, and the detection results were analyzed using statistical method. The test results showed, in the study area, phenolic endocrine disruptors were generally detected in various media. Many media distribution:organisms> sediments> water. Principal component analysis results showed that the residue situation of p-Tert-butyl phenol, bisphenol A, tert-octylphenol, nonylphenol in seawater, sediments and organisms, could basically reflect the residual situation of six kinds of phenolic endocrine disruptors in these three medias. Cluster analysis showed phenolic endocrine disruptors existed in organisms according to the law of nutrition enrichment.