Screening And Identification Of Bacillus Sp. Strain Producing Alginate Lyase And Fermentation Conditions

Marine microbial normal living environment is the main sea water, compared with the land, the marine environment has high pressure, high salt, low temperature and dilute nutrition characteristics, which makes the marine microorganisms and terrestrial microorganisms in physiological properties and physicochemical properties with respect to produce bigger difference.Because the marine microbial special physiological characteristics, which produces a unique structure, functional diversity of active substances, and the development and utilization of these marine active substances produced by microorganisms, make it better serve human has become the focus of today’s world.This paper mainly collected from decaying keep with pathologies as sample, selected from alginic acid decomposing enzymes isolated from marine microbe strains1and strain2, at the same time of strain1and2growth characteristics of strains, fermentation and the optimal culture conditions were studied.In this thesis, using the solid-liquid culture strains were activated alternately, and solids separation plate medium strains were isolated and purified to facilitate observation and part of the post-test operation. During the test, the two strains:1strains and2strains were alginate-degrading enzyme to produce strains. In this paper, in order to determine the marine microorganisms of alginic acid decomposing bacteria strain1and2strains producing better culture conditions, using the DNS method for the determination of fermentation enzyme activity, experimental data show that, the optimal culture conditions for:7%inoculation quantity, medium quantity30mL, culture temperature26℃; the best pH value of medium6; culture time4d; the optimal culture medium was made:sodium alginate concentration is0.4%; corn slurry concentration is1%; mixed carbon source concentration (sucrose concentration) was1%, the optimal concentration of NaCl was3%.