Startup And Bioaugmentation On Operation Of Biohydrogen Production Reactor

Fermentation hydrogen production technology not only can achieve thewaste resource utilization, reduce the pollution of the environment, but also canobtain the new alternative energy of hydrogen. In order to improve the ability ofbiohydrogen production reactor to produce hydrogen, the startup andbioaugmentation of biohydrogen production reactor was operated with molasseswastewater as substrate in the CSTR reactor. So as to the study can promote theindustrialization process of reactor, and provides a solution for the developmentof alternative energy.The results showed that after30days, the process performed steadily underthe condition of influent COD concentration at3000mg L-1, hydraulic retentiontime at8h, and temperature of (35±1)℃. The maximal biogas production ratecould reach at6.3L d-1and the maximal hydrogen production rate at754mL d-1under this condition. The reactor showed a stable COD removal efficiency of20%. Effluent pH ranged from4.3to4.5. The total amount of ethanol and aceticacid was1650mg L-1, accounting for80%of the total liquid products, which canbe attributed to ethanol-type fermentation. The settlement performance of matureactive sludge was good. The settling velocity (SV30) was25%. On the contrast, itwas only47%in the initial stage.3strains were isolated from productionhydrogen fermentation sludge by the Hungate anaerobic roll tube method.Bioaugmentation of CSTR reactor was operated, the results showed that theaddition of efficient hydrogen production bacteria could shorten system start timeat system startup phase, the average biogas production capacity was improved32.5%and42.5%respectively, when the inoculation quantity of efficienthydrogen production bacteria were3.3%and4.9%. Moreover, when influentCOD concentration was increased to5000mg L-1, reactor was operated in goodcondition. It showed that bioaugmentation increased the system’s ability to resist impact load, and enhance the running stability of the system.