Synthesis And Characterization Of Pnipam Thermal-Sensitive Nanohybride Drug Carrier

Synthesis of nanohybride materials has become a hot topic the in recent years, particllarly in the combination of functional polymer withnano-materials. The applications of the nanohybride materials in biochemical and biomedicine have been paid more attention based on their great biocompatibility. Two kinds of materials have been synthesized in our research as the nano-carriers for the drug delivery, including MWCNT-polymer and GO-polymer.Firstly, the thermo-sensitive polymer was created along MWCNTs by an one-pot atom transfer radical polymerization reaction with poly(ethylene oxide) macromonomer and N-isopropylacrylamide monomer.The nanostructure and morphology were characterized by different techniques. Based on its biocompatibility and properties, the nanobybride materials were applied for the drug release as the nanocarriers. The results of it displays that the drugs released from MWCNT-polymer differs from the test temperatures.Secondly, the functional GO grafted with the thermal-sensitive polymer nanoparticles were synthesized. The nanostructure and morphology have been proved by different techniques. In addition, Cell culture experiment with themouse osteoblast-like MC3T3-E1cells indicates that the synthesized GN-PNP hybrids have satisfied biocompatibility. Our study indicates that this thermo-sensitive GO-PNP hybrid, when considering the drug loading and release comprehensively, has better performance than both PNPs and GO and thus can be used as a novel nanocarrier for the temperature-controllable drug release. The GO-PNP hybrids,with and withoutADR, were utilized to kill cancer cells.The result implys that not only the GO-PNP hybrid with ADR has obvious effect on killing cancer cells, but also its drug delivery performance is obviously better than both GO and PNPs.These two kinds of nanohybride materials shows satisfied drug delivery performance in our research.It is expected that this new hybrid material based on MWCNT, GO and thermal-sensitive polymer will have great potentials for in vivo applications such as to kill target cancer cells by modifying with specific antibodies.