Chitosan Hydrogel Synthesis And Its Applications In Biomedicine Combinated With Superwettability

Hydrogel with high moisture contents, well biocompatibility and mechanicalstrength，is widely used in tissue engineering and biomedical fields. There are manyspecies of hydrogels, which preparation methods include physical crosslinking,chemical crosslinking, light-crosslinking and so on. The use of hydrogel is widelyextended to various fields, for example: hydrogel acts as biological scaffolds in tissueengineering. Moreover, it has a high efficiency on completing the functions of releaseand packaging as the supporter of drug release and cell encapsulation. Depending onthe materials, the species used to prepare the hydrogel are varied, including nativehydrogel and artificial hydrogel. The natural chitosan-hydrogel has gained widelyattentions because of the excellent biocompatibility, nontoxicity and low-cost.In this paper, the authors prepared chitosan-hydrogels through the reactionbetween chitosan and genipin as cross-linking agent. The tissue engineering scaffoldswith porous structure is prepared through freeze-dried processing. After UVirradiation treatment, ultrafast spreading and permeating effect were obtained. Theprepared chitosan scaffolds that had ultrafast spreading and permeating effect wereused in cell cultures. The results of experiments showed that it was easier for cell toenter into the scaffold with ultrafast spreading and permeating effect than that had notultrafast spreading and permeating effect. In addition, the adhesion and proliferationof cells were also improved in the scaffold resulted by the coefficient of UVirradiation and ultrafast spreading and permeating effect. However, it would take along time to make the cells immerse the inside of tissue engineering scaffolds. It is afeasible method that this experiment provides for shortening the treatment cycle intissue engineering.According to the droplet was spherical on the superhydrophobic surface, we prepared the chitosan hydrogel sphere with magnetism. After freeze-dried，the loosetexture and porous structure of the surface of chitosan hydrogel can tightly grasp thewater-soluble proteins. The magnetic chitosan hydrogel sphere act as a core, thewater-soluble proteins around it was also performed as a sphere. By moving themagnetic field, the water-soluble proteins can be drived as will. And it remains kindsof fluorescent-protein patterns at the superhydrophobic substrate. This technologyprovides a new way of simple operation and low cost for complex operation and highcost protein chip preparation and protein graphic operation.