Study On The Structure Research And Immunological Activity Of Polysaccharide From Elaeagnus Angustifolia L. In Xinjiang Province

Object: To extract and purify esearch polysaccharides from Elaeagnus angustifolia L.in XinjiangProvience. Angustifolia polysaccharides were isolated and purified homogeneous components, butalso the structure and immune activity of homogeneous components were carried out.Methods:1.The DM-18macroporous resin,DEAE-52cellulose column chromatography, G-100column chromatography and dialysis purification to get homogeneous components. Thesepolysaccharides were identified homogeneous components by using a UV-visiblespectrophotometry, TOYOPEARLHW-55F gel chromatography and specific rotation.2. The three homogeneous components were identified structures on the molecular weightdetermination, monosaccharide composition analysis and infrared analysis.3. Homogeneous components of three angustifolia polysaccharide immune activity were carriedout on immune organ weight of mice to study the function (carbon clearance rate), monocytemacrophages, mouse serum hemolytic hormone testing, and delayed-type hypersensitivity (DTH)test.Results:1. Optimum adsorption conditions of DM-18macroporous resin were: the sampleconcentration of2.0mg/mL, the sample solution pH of7.0, the sample rate of1.5BV/h, thesample volume was3.0BV. Optimum desorption conditions: eluent ethanol concentration of35%,the elution rate of1.0BV/h, the amount of eluent to4.0BV, ethanol eluent pH was7.0.2. Through the DEAE-52cellulose column to get three main components, and G-100columnchromatography were further isolated to get the angustifolia polysaccharide components. Thesepolysaccharides have been disposed with dialysis impurity. To Identificate the six of homogeneouscomponents, three methods were used. Firstly, UV-visible spectrophotometry in the wavelengthrange of200~400nm were no absorption, which indicated that six components were free ofproteins and nucleic acids. Secondly, gel chromatography and specific rotation were used toidentificate the homogeneous components, the results showed that PEA-1, PEA-3, PEA-6angustifolia polysaccharide group were homogeneous components.3. The molecular mass of three homogeneous components were:873747,286169and14656. Gaschromatographic analysis was used to get the molar ratio of the homogeneous components. Themonosaccharide composition of the homogeneous components were as follow. Firstly, PEA-1wascomposed of rhamnose, arabinose, xylose, glucose, mannose, galactose, and the monosaccharidemolar ratio was1.3:0.56:0.26:1.0:1.0:2.26;Secondly, PEA-3was composed of rhamnose, mannose,glucose, galactose, and the monosaccharide molar ratio was1.86:0.99:6.27:5.68;Thirdly, PEA-6was composed of rhamnose, xylose, mannose, glucose, galactose, and the monosaccharide molarratio was6.4:0.33:1.05:6.0:3.19.The three components all contained polysaccharides characteristicabsorption peaks by infrared spectroscopy. The PEA-1contained in α-glycosidic bond, and was mainly furanose; the PEA-3contained in β-glycosidic bond, and was mainly pyran; The PEA-6contained in α-glycosidic bond, and was mainly furanose.4. Angustifolia polysaccharide verified under homogeneous components of mice withcyclophosphamide role in immune regulation.Conclution: This study conducted in-depth research of angustifolia polysaccharide to obtain thehomogeneous components, and the structure of the homogeneous components which were alsoidentified have immunological activity.