Study On Complex Nanocarriers Containing Poly(Ascorbyl Acrylate) For Tumor Inhibition Drug Carrier

As we all kwon that vitamin C is an important nutrent and powerful antioridants to protect the body from free radicals against. Recent studies have shown that the most significant chemical properties of vitamin C was inhibition of tumor cell growth and the application prospects was optimistic. Mechanism of tumor inhibition, vitamin C acted as a pro-drug to deliver hydrogen peroxide to tissues. Based on tumor inhibition of vitamin C, in this article, the breakthrough was that a complex nanocarriers containing poly (ascorbly acrylate) was invented and studyed on the complex nanocarriers for tumor inhibition drug carrier. Specifically, the mainly works are as follows:1. First of all, poly (lactic acid)-block-poly (ascorbyl acrylate)(PLA-block-PAAA) served as one kind of nanomaterials for composition of the complex nanocarriers was prepared by atom transfer radical polymerization and ring-opening polymerization;2. We successfully synthesized a novel type of amphiphilic block copolymers polylactide-poly(ethylene glycol) with maleimide (PLA-PEG-Maleimide). Two types of polymers were prepared to form the the complex polymer micells. It was surprising that the stability of the complex polymer micells is perfect. Furthermore, we characterized the complex polymer micells in detail;3. Cytotoxicity of the PLA-block-PAAA/PLA-PEG-Maleimide complex polymer micells were detected by MTT assay. The analysis of Human vein endothelial cells viability suggested that the polymeric micelles from PLA-block-PAAA had low cell toxicity, and it has the potential to be used as drug delivery carrier in vivo;4. Cytotoxicity on tumor of the PLA-block-PAAA/PLA-PEG-Maleimide complex polymer micells were detected by MTT (3-(4,5-Dimethylthia-zol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The results demonstrated that the polymer micells own great tumor inhibition property, and it has the potential to be used as drug delivery carrier in vivo; Furthermore, we compare polymer micells with vitamin C and the complex polymer micells get the edge on tumor inhibition;5. Paclitaxel (PTX) was embedded into the PLA-block-PAAA/PLA-PEG-Maleimide complex polymer micells, and studied stability of the complex polymer micells. Scavenging effects on DPPH (1,1-Diphenyl-2-picrylhydrazyl radical2,2-Diphenyl-l-(2,4,6-trinitrophenyl) hydrazyl) radicals, the release behavior, encapsulation rate and drug loading of the polymer micells;6. Cytotoxicity on tumor of the PLA-block-PAAA/PLA-PEG-Maleimide complex polymer micells were detected by MTT assay. The results proved that the polymer micells with paclitaxel inhibit the growth of tumors better. The main reason is poly (ascorbyl acrylate)(PAAA) functioned as an alleviator on normal cells and a synergistic effector on cancer cells with paclitaxel.