Strengthening Microbial Agent About Sludge Composting Microbial Community Structure Based On PCR Technology

Sludge is the largest city byproducts generated during wastewater treatment. It is also arenewable resource.Municipal Sludge Compost is a very important approach.But traditionalsludge compost contains a lot of cellulose, lignin and it cause long-composting cycle.It hasimportant significance to shorten the fermentation cycle sludge compost, reduce the sludgecompost fermentation period, accelerate the decomposition rate of urban sewage sludge.Many studies have shown that,inoculating microbes can effectively shorten thecomposting cycle, accelerate the decomposition of organic matter and extend thehigh-temperature phase,because it can improve the traditional sludge compost.This paperaims at to built a set of sludge degrading bacteria F4,which is built under restrictive cultureconditions.Its community composition analysis is by molecular biology.F4is also applied insludge compost system.Specific studies are as follows:Screening of sludge degradation thermophilic bacteria F4.Use dry sludge as therestrictive culture factor, select bacteria from environment sample or pile of manuresource,get three kinds of combinations of proportion, C1, C17, C18. Extract bacteria wasinoculated in a ratio of5%, cultured with shaking at50°C,150rpm and passaged every48hwith amount of5%, a total of5generations.And measued the organic matter content duringevery48h.Rescreening.MixingC1,C17,C18with D which has already been selected by thelaboratory,at different proportions.F1, F2, F3, F4, F5was the final rescreeningresult.Rescreening is also at the same proportion of5%of vaccinated inoculated in freshmedium, temperature60℃, shaking speed150rpm, passaged once every48h.At last F4isselected by organic matter content in each generation at48h,which has stable organiccompound degradation effectively. Part of the growth characteristics of measuring F4complex bacteria, such as pH and OD values,have been measured.F4has biologically activeand the normal decomposition of organic matter.By constructing molecular biology techniques16SrDNA clone library, analyzed thecolonies composed of microbial compound F4. The16SrDNA bacterial clone libraryconstruction showed that, Lactobacillus thermophilus accounted for8.43%of the storagecapacity,3.61%Bacillus smithii accounted library capacity,3.61%Anaerobic thermophileaccounted library capacity, Bacillus coagulans accounted for3.61%of storage capacity,Geobacillus thermoparaffinivorans accounted for3.61%of the storage capacity, Geobacillusthermoglucosidasius storage capacity of1.2%, Geobacillus stearothermophilus storagecapacity of1.2%. Actinomycetes16SrDNA clone library construction resulting microbialcommunity composition, Bacillus coagulans storage capacity of90%,3.61%Bacillussmithii accounted for storage capacity, the dominant bacteria of Bacillus coagulans.Application of complex bacteria sludge compost. F4was applied in sludge compost atthe proportion of5%. Microbial compound F4adjusted the community srtucture,strengthenedthe synergy,extend the high-temperature heating period,decomposted the organic effectively and the moisture content decreased rapidly.This paper screened complex bacteria by restriction culture techniques.complexmicrobial strains has been studied through molecular biology.It was a theoretical guidancefor sludge disposal resources.