The Screening And Identification Of Differential Expression Proteins In Cholesterol-lowering Lactobacillus Plantarum

It is well known if the level of cholesterol is too high it will be harm to human body health,it is the main factor to induce cardiovascular disease, the cardiovascular disease has become oneof the world’s five leading cause of death, so it is the international hot subject about how toreduce serum cholesterol content. The cholesterol-lowering ability of Lactobacillus plantarumhave been recognized by many scholars at home and abroad, but its degradation mechanism isnot yet clear. This study we researched the differential expressed proteins of Lactobacillusplantarum of different cholesterol-lowering ability by proteomics technology. It can providescientific basis for the cholesterol-lowering mechanism of Lactobacillus plantarum.In this study, we test the cholesterol-lowering ability of Lactobacillus plantarum M1andLactobacillus plantarum UVs29, and then protein was extracted from the Lactobacillusplantarum M1and Lactobacillus plantarum UVs29. After sample proteins were separated bytwo-dimensional polyacrylamide gel electrophoresis, silwer stained gel imaging to get thedifferential expression proteins, then, the spots of the differential expression proteins wereanalyzed by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry(MALDI-TOF-MS) and the search of NCBI database, and then were analyzed online throughbioinformatics.Electrophoretogram of Lactobacillus plantarum M1and Lactobacillus plantarum UVs29were obtained by2-DE,24differential expressed proteins were separated and identified byMALDI-TOF-MS, and results showed that these proteins are30S ribosomal protein S9,enolase,30S ribosomal protein S4, adenine phosphoribosyltransferase, heat shock proteinHsp20,elongation factor P,fructose-bisphosphate aldolase,molecular chaperone DnaK,adenylatekinase,30S ribosomal protein S3,NADH peroxidase,serine hydroxymethyltransferase,orotatephosphoribosyltransferase, TetR family transcriptional regulator,Ribosome-recycling factor,unnamed protein product,UTP-glucose-1-phosphate uridylyltransferase,6-phosphogluconatedehydrogenase, enoyl-ACP reductase,Elongation factor Tu,S-ribosylhomocysteine lyase,transcriptional regulator,uracil phosphoribosyltransferase, ribose-phosphate pyrophosphokinase,50S ribosomal protein L1.In this study, we first explore two strains of Lactobacillus plantarum of different cholesterol-lowering ability by proteomics. Two-dimensional gel electrophoresis and massspectrometry were used to separate and identify the24differential expressed proteins. Thendiscuss the relationship between these differential expressed proteins and cholesterol-loweringability of Lactobacillus plantarum， however, it need to verify these proteins and theirrelationship with cholesterol-lowering ability in the future. This experiment had very importantguiding meaning of ascertain the cholesterol-lowering mechanism of Lactobacillus plantarum.