Study On The Enzyme Hydrolyzation, Decoloration And Separation Of Soybean Polypeptides

The soybean polypeptides are a compound with certain molecular which are obtained from hydrolysis of soybean protein.Compared with soybean protein,soybean polypeptides have more superior physical and chemical properties,also bioactivities.There are good prospective of exploitation and application in food industry and medical works.Soybean dregs are used as materials in this paper.The paper studied the enzyme activities from the stain under the solid-state fermentation,showed that the best situation for enzyme activities is temperature for 50℃,pH 7.2.Selected the most optimum conditions for solid-state fermentation of soybean dregs,studied the optimum process of soybean polypeptides enzyme hydrolysis,decided the optimum conditions as 50℃,feed to water 1:6,hydrolyzing for 11 h by orthogonal experiment,the soybean polypeptides trasferring rate was 69.6%(w/w).Gel chromatography showed that there were 87.61% of soybean polypeptides with molecular weight between 1000 D-6000 D.Active carbon was used for decolorization,the optimum conditions for the decolorization were active carbon 2%,temperature 50℃,pH5,decolorizing 3 h,the decoloration rate reached 75.2%. Membraneseparation was further used for soybean polypeptides.The composition of membrane separation system was identified,also the process of the membrane separation and operation of the main parameters.