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Mtdna Lineages And Initial Studies On Origin Of Hainan Gelong

Posted on:2011-04-06Degree:MasterType:Thesis
Country:ChinaCandidate:K YangFull Text:PDF
GTID:2190330335484957Subject:Biochemistry and Molecular Biology
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The topic about origin and differentiation of people being is continuously interested for people.People can solve the problem with the latest technical means as deeper knowledge and research on biological, the appearance of technologies, such as computer, cross-application of the various disciplines.The main purpose of this study is the molecular anthropology analytics detailed of the maternal genetic structure of Gelong and investigate the origin of Gelong from the molecular level first time.Research ideas:we make a classification (also called haplogroups) on every people with the information of HVS-â… region and coding region of mtDNA which got from corresponding individual by experiment of molecular anthropology. Using a range of software about molecular anthropology and bioinformatic, we discussed about the differences of molecular genetic structure in detail between Gelong people and other ethnic groups related.In method,firstly we make a sequencing of the HVS-I of mtDNA. In the same time,we exzambled the information of sites (23 sites in this study) on the coding-regions with the Snap-shot technology. From that, we classed the indivilual to the haplogroup-type. Compositing the results on two section we detected, we classed every individual to a haplogroup according to the latest phylogenetic tree of mitochondrial haplogroup (Figure 1) for the East Asian populations constructed by Kivisild and a simplified phylogenetic tree of the East Asian population.According to the result of haplogroup,we maked a statistic on the frequency of distribution about the haplogroup.Using the software of SPSS15.0 we maked it a scatter distribution under two dimensional to analyze the distribution pattern of Gelong groups in all groups from the East Asian. We make networks to observe the relationship between every group related according to the information of HVS-I of every individual related under the same haplogroups special.We make an admixture calculation to analyze the ratio of contribution of parental which may make a role in contribution to Gelong group on network or PC analyze.The results of haplogroup of Gelong:M* (20.91%), B (17.27%), F (13.64%) and R (13.64%).The result of PC and network:the genetic structure of Gelong is similarrest to the Gelao which belong to the same lingual branch which called kadai.The genetic structure of Gelong is also influenced by Han Chinese and Hlai. Parental contribution index of the maternal inheritance:Gelao 0.376+/-0.015,Han Chinese 0.317+/-0.014,Hlai 0.307+/-0.014.Conclusion:the main crowd of Gelong group should be from the Gelao groups. The conclusions is consistent with the result of the paternal genetic structure early.
Keywords/Search Tags:Gelong, mtDNA, Gelao, Matrilineal genetic structure, Origin
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