Wild Toot Salt In Bacteria Isolated And Cultured And The Nature Of The Ancient Rhodopsin Protein

Extreme halobacteria are wide spread throughout the world, China also have abundant halophilic bacteria resource. The proton pump bacteriorhodopsin(bR) is the only protein found in the purple membrane of Halobacterium salinarum. Its chromophore, retinal, is bound via a protonated Schiff Base to the e -amino group of a lysine residue in the 26,000-mol wt protein. Photon absorption by the chromophore, all-trans retinal, initiates a cycle of reactions that result in vertical proton translocation across the membrane. bR is the simplest known light energy-converting systems. It is very small and requires only light and a lipid bilayer to generate an electrochemical ion gradient, which can be used by the cell for ATP synthesis and other metabolic processes. Such an unique photoelectric response make bR the most promising biological material in many fields.It was found that there are many bacteriorhodopsin-like proteins belong to the archaerhodopsin family distribute in the extreme environment. Some of them have photo reaction and photoelectric characteristic similar with bR. We don't know too much about them. More information about their structure and function relationship will be meaningful in understanding the molecular mechanism of ion pump and conformation alteration during ion transport and sensory transduction.In this paper, we report screening strains from samples collected from Ebi lake and Altun moutain Aqik lake, Xinjiang Province, and got 60 clonies from them. We choose 8 of them for further investigation and confirmed that they all belong to the Halobacteriaceae genus and through comparasion of several minute diffrerence medium we had the optimal medium for their growth.. Through this procedure we had established a set of methods for screening clonies from wild environment.and isolation and purification of these archaerhodopsin. Furthermore we used PCR method to identify their DNA sequence. A fine base had been set up for further investigetion.Today more intension had been focused on biological materials due to the deficiency of resource throughout the world. The excellent properties of bR and other archaerhodopsins had bring considerable interest in utilizing them in energy-converting system, information storage, nano-sensors and other fields. Modem molecular biological techniques and chemical modification can even enlarge the field of application. Our research provided a enlargable platform for development and application of halophilic bacteria.